渤海沉积物中产甲烷途径及产甲烷菌群落特征  

Methanogenic pathways and methanogen communities in the sediments from Bohai Sea

作　　者：许晓晴 陈烨 甄毓[1,2,3] 米铁柱 李晶[4] 刘昌岭 XU Xiaoqing;CHEN Ye;ZHEN Yu;MI Tiezhu;LI Jing;LIU Changling(Key Laboratory of Marine Environment and Ecology,Ministry of Education,Ocean University of China,Qingdao 266100,China;Laboratory for Marine Ecology and Environmental Science,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China;College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China;Qingdao Institute of Marine Geology,China Geological Survey,Qingdao 266237,China)

机构地区：[1]中国海洋大学海洋环境与生态教育部重点实验室,青岛266100 [2]青岛海洋科学与技术国家实验室,海洋生态与环境科学功能实验室,青岛266237 [3]中国海洋大学环境科学与工程学院,青岛266100 [4]中国地质调查局青岛海洋地质研究所,青岛266237

出　　处：《海洋地质与第四纪地质》2022年第3期50-61,共12页Marine Geology & Quaternary Geology

基　　金：国家自然科学基金“黄东海典型海域底边界层磷的生物地球化学过程研究”(42130410);国家自然科学基金“中国近海氨氧化微生物氨氧化及反硝化过程研究”(41806131);青岛海洋科学与技术国家实验室鳌山科技创新计划项目(2016ASKJ02)。

摘　　要：全球甲烷排放主要来源于厌氧环境中产甲烷菌的代谢活动。采集渤海典型站位沉积物样品,测定了沉积物中甲烷和硫酸盐含量。通过提供不同类型产甲烷底物进行培养,分析了各样品中甲烷产量;通过高通量测序,分析了产甲烷菌群落特征。结果表明,渤海沉积物中产甲烷途径以甲基营养型为主,同时具有H_(2)/CO_(2)还原型途径。同一站点随着深度的增加,甲烷产生量逐渐减少,产甲烷速率相应降低。古菌群落以Crenarchaeota、Asgardaeota和Nanoarchaeota为主,产甲烷菌Ca.Methanofastidiosales占据优势地位。本研究为全面了解产甲烷菌在海洋生境中产甲烷过程提供了重要参考。Mostly,the global methane emission comes from the metabolic activities of methanogens,which occurs in anaerobic environment.Based on the samples collected from typical stations in the Bohai Sea,we measured the concentrations of methane and sulfate in the sediments.The methane concentration in each sample was analyzed with different methanogenic substrates cultivation.The methanogen communities were measured by high-throughput sequencing techniques.The results indicated that the mainly methylotrophic pathway is methylotrophic methanogenesis,along with the hydrogenotrophic methanogenesis.Methane production decreased gradually with the depth increasing at the same station,and methane production rate decreased correspondingly at the same time.The archaea communities were dominated by Crenarchaeota,Asgardaeota and Nanoarchaeota,and Ca.Methanofastidiosales was the dominated methanogens.The results provide useful information for understanding the methanogenesis of methanogens in marine ecosystem.

关 键 词：产甲烷菌 沉积物 甲基营养型 H_(2)/CO_(2)还原型 渤海 

分 类 号：P736.4[天文地球—海洋地质]