钛铁试剂对低温胁迫及恢复后秋茄幼苗光合生理特性的影响  

Effect of Tiron on Photosynthetic Physiological Characteristics of Kandelia obovata Seedlings under Low Temperature Stress and Recovery

作　　者：潘晓娇 郑春芳 刘伟成[3] 陈继浓 丁文勇[3] PAN Xiao-jiao;ZHENG Chun-fang;LIU Wei-cheng(School of Design and Digital Arts,Zhejiang Industry and Trade Vocational College,Wenzhou,Zhejiang 325000;College of Life and Environmental Science,Wenzhou University,Wenzhou,Zhejiang 325035;Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource,Zhejiang Mariculture Research Institute,Wenzhou,Zhejiang 325005)

机构地区：[1]浙江工贸职业技术学院设计与数字艺术学院,浙江温州325000 [2]城镇水污染生态治理技术国家地方联合工程研究中心,温州大学生命与环境科学学院,浙江温州325035 [3]浙江省海洋水产养殖研究所,温州海洋研究院,浙江省近岸水域生物资源开发与保护重点实验室,浙江温州325005

出　　处：《安徽农业科学》2022年第12期120-123,140,共5页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(32071503);温州市科技计划项目(N2020009)。

摘　　要：[目的]探讨钛铁试剂对秋茄幼苗的抗寒性调控作用。[方法]以秋茄幼苗为试材,研究喷洒10 mmol/L钛铁试剂对低温胁迫及恢复后植株叶片光合和叶绿素荧光参数、抗氧化系统以及细胞膜透性的影响。[结果]钛铁试剂能显著提高低温胁迫及恢复后秋茄幼苗叶片净光合速率(P_(n))、气孔导度(G_(s))、PSII最大光化学效率(F_(v)/F_(m))、实际光化学效率(Φ_(PSII)),光化学猝灭系数(q_(P)),抑制非光化学猝灭系数(NPQ);钛铁试剂也能增加短期低温胁迫及恢复后秋茄幼苗叶片的超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)活性,抑制相对电导率以及MDA含量积累。[结论]钛铁试剂能有效改善植株抗氧化系统,提高植株的光合作用,从而增强秋茄幼苗的抗寒性。[Objective]In order to explore the regulative role of tiron on cold tolerance of Kandelia obovata seedlings.[Method]An experiment was carried out to analyze the effects of 10 mol/L tiron on parameters of gas exchange and chlorophyll fluorescence,antioxidant system and relative electrical conductivity in K.obovata seedling to low temperature stress and recovery.[Result]The results showed that tiron significantly increased leaf net photosynthetic rate(P_(n)),stomatal conductance(G_(s)),photochemical efficiency of photosystem II(F_(v)/F_(m)),actual photochemical efficiency(Φ_(PSII)),photochemical quenching coefficient(q_(P))in K.obovate,while significantly decreased leaf non-photochemical quenching coefficient(NPQ).In addition,tiron increased the activities of superoxide dismutase(SOD),peroxidase(POD)and ascorbate peroxidase(APX),and alleviated the oxidative damage in leaves of K.obovata by reducing cell membrane permeability(relative electrical conductivity)and lipid peroxidation(malondialdehyde,MDA).[Conclusion]The study indicates that tiron could effectively improve the antioxidant system,increase leaf photosynthesis,and enhance cold tolerance of K.obovate seedlings.

关 键 词：红树林 秋茄 钛铁试剂 低温胁迫 光合作用 酶活性 

分 类 号：S796[农业科学—林木遗传育种]