2012—2018年长春市手足口病非肠道病毒A组71型肠道病毒VP1基因特征分析  

Characteristics of VP1 gene of non-enterovirus A71 causing hand,foot and mouth disease in Changchun City,Jilin Province,China from 2012 to 2018

作　　者：张智丽 李晨光 张兴国[1] 孙宇[1] 崔薇[1] 乔凤娟[1] 孙炳欣[1] ZHANG Zhi-li;LI Chen-guang;ZHANG Xing-guo;SUN Yu;CUI Wei;QIAO Feng-juan;SUN Bing-xin(Changchun Center for Disease Prevention and Control,Changchun 130033,Jilin Province,China)

机构地区：[1]长春市疾病预防控制中心,吉林长春130033

出　　处：《中国生物制品学杂志》2022年第3期304-311,315,共9页Chinese Journal of Biologicals

基　　金：吉林省卫生与健康技术创新项目(其他)(2018J008)。

摘　　要：目的 分析2012—2018年长春市手足口病(hand,foot and mouth disease,HFMD)非肠道病毒A组71型(enteroviruses A71,EV-A71)肠道病毒VP1基因特征,探讨其流行规律,为及时调整防控策略提供参考。方法 收集2012—2018年长春市医疗机构报告的HFMD临床诊断病例样本,选取非EV-A71型肠道病毒对其VP1区扩增并测序,比对构建系统进化树,进行核苷酸同源性及氨基酸变异位点分析。结果 2012—2018年长春市HFMD病例中共获得83条柯萨奇病毒A组16型(coxsackievirus A16,CV-A16)序列、30条柯萨奇病毒A组6型(CV-A6)序列和10条柯萨奇病毒A组10型(CV-A10)序列,其中CV-A16序列全部属于B1亚型,除2012年1条和2018年7条序列属于B1a亚型,其他均属于B1b亚型,各株间核苷酸序列同源性为88.2%~100.0%。2012—2018年长春市HFMD病例中CV-A6毒株相对集中,30条序列均属于D3亚型,与D3亚型参考株的同源性为92.8%~98.1%,各株间同源性为91.6%~99.8%;CV-A10毒株相对集中,10条序列均属于D2亚型,与D2亚型同源性较高为96.4%~98.4%,各株间同源性为96.4%~100.0%;CV-A16毒株中存在14个氨基酸位点变异,有12株发现在第14位点上发生N天冬酰胺→S丝氨酸(N14S)的变异,其中9株同时存在L215P变异。有9株存在M23L变异,其中8株为B1a亚型。结论 2012—2018年长春市HFMD病例中CV-A16流行株均属于B1亚型,且同时存在B1b和B1a亚型,B1b为主要优势型别,毒株存在14个氨基酸位点变异,其中N14S和L215P可能成为稳定流行位点。CV-A6和CV-A10毒株相对集中,分别为D3和D2亚型,同源性较好。加强对非EV-A71型HFMD病原的检测,对HFMD疫情的防控具有重大意义。Objective To analyze the characteristics of VP1 gene of non-enterovirus A71(non-EV-A71) causing hand,foot and mouth disease(HFMD)in Changchun City,Jilin Province,China from 2012 to 2018,investigate the epidemic regularity of the disease and provide a reference for adjusting the strategy of control and prevention promptly. Methods The specimens of cases of HFMD diagnosed clinically were collected from medical institutes in Changchun City from 2012to 2018,from which the VP1 regions of non-EV-A71 were screened and sequenced to construct the phylogenetic tree and analyze the homology of nucleotides and the variation site of amino acids. Results A total of 83 coxackievirus A16(CVA16)sequences,30 CV-A6 sequences and 10 CV-A10 sequences were obtained from the specimens of cases of HFMD in Changchun from 2012 to 2018. All the CV-A16 sequences belonged to subtype B1. However,one sequence in 2012 and seven ones in 2018 belonged to subtype B1a,while the others belonged to subtype B1b. The homologies of nucleotides of the strains were 88. 2% ~ 100. 0%. The CV-A6 strains were relatively concentrated in cases of HFMD,in which all the30 sequences belonged to subtype D3,with a homology of 92. 8% ~ 98. 1% to reference strain of subtype D3,while the homology between various strains was 91. 6% ~ 99. 8%. The CV-A10 strains were relatively concentrated,in which all the10 sequences belonged to subtype D2,with a homology of 96. 4% ~ 98. 4% to the reference strain of subtype D2,while the homology between various strains was 96. 4% ~ 100. 0%. There were 14 amino acid variations in CV-A16 strains,including the variation from N asparagine-S serine at site 14(N14S)in 12 strains,in 9 of which the variations of L215P were observed at the same time. Variations of M23L were observed in 9 strains,of which 8 belonged to subtype B1a.Conclusion All the CV-A16 epidemic strains in Changchun City from 2012 to 2018 belonged to subtype B1,while subtypes B1b and B1a were observed at the same time,of which subtype B1b was dominant. There were 14 amino aci

关 键 词：手足口病 肠道病毒 VP1蛋白 基因型别 

分 类 号：R373[医药卫生—病原生物学]