代谢流分析在CHO细胞代谢研究中的应用进展  

Progress in applications of metabolic flux analysis in metabolism of CHO cells

作　　者：彭婷(综述) 梅文枫(审校) PENG Ting;MEI Wen-feng(Institute of Immunotherapy,Fujian Medical University,Fuzhou 350122,Fujian Province,China)

机构地区：[1]福建医科大学免疫治疗研究院,福建福州350122

出　　处：《中国生物制品学杂志》2022年第3期363-368,378,共7页Chinese Journal of Biologicals

基　　金：福建省自然科学基金资助项目(2019J05074)。

摘　　要：CHO细胞可进行正确的翻译后修饰,如蛋白折叠、糖基化,且其表达的重组蛋白可分泌至胞外,更便于后续纯化,已成为抗体药物的首选表达宿主。代谢流分析(metabolic flux analysis,MFA)又称代谢通量分析,可通过同位素示踪剂绘制胞内流动的代谢途径来量化这些代谢表型,其综合底物吸收及产物形成速率、生物合成、化学计量反应、中间代谢物的生物合成等数据,可为重新构建细胞代谢提供关键的量化信息。13C-MFA是以13C标记实验为基础的MFA,已成为研究CHO细胞工程改造后的变化及指导培养基和生物工艺优化的重要工具之一。本文就13C-MFA流程及近年MFA在CHO细胞代谢研究中的应用进展作一综述。CHO cells has become the top choice of host for expression of antibody drugs due to correct post-translational modification such as protein folding and glycosylation,and the expressed recombinant protein may be secreted to excellular region and easy to further purify. Metabolic flux analysis(MFA) is a powerful technique for quantification of metabolic phenotypes by drawing the metabolic pathway of intracellular metabolic fluxes with isotope tracer,of which the data on integrated substrate absorption,product formation rate,biosynthesis,stoichiometric reaction as well as the biosynthesis of intermediate metabolites may provide key quantification information for remodeling of cell metabolism.13C-MFA has become an important tool for research on change of engineered CHO cells and guiding the optimization of medium and bioprocess. This paper reviews the process of13C-MFA as well as the progress in applications of MFA in metabolism of CHO cells in recent years.

关 键 词：代谢流分析 CHO细胞 同位素标记 

分 类 号：Q819[生物学—生物工程]