黄杞叶提取物对小鼠造血系统辐射损伤的防护作用  

Protective effects of Engelhardia roxburghiana Wall.leaf extract on radiation injury of the hematopoietic system in mice

作　　者：吴少华 常华杰 勾文峰 郭江红 宁洪鑫 李祎亮[2] 侯文彬 Wu Shaohua;Chang Huajie;Gou Wenfeng;Guo Jianghong;Ning Hongxin;Li Yiliang;Hou Wenbin(Major of Traditional Chinese Medicine,Graduate School,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine,Institute of Radiation Medicine,Chinese Academy of Medical Sciences,Tianjin 300192,China)

机构地区：[1]天津中医药大学研究生院,天津301617 [2]中国医学科学院放射医学研究所,天津市放射医学与分子核医学重点实验室,天津300192

出　　处：《国际放射医学核医学杂志》2022年第2期92-102,共11页International Journal of Radiation Medicine and Nuclear Medicine

基　　金：中国医学科学院医学与健康科技创新工程(2016-I2M-3-022);中央高校基本科研业务费专项(3332020057);天津市科技计划项目(18ZXXYSY00110)。

摘　　要：目的研究黄杞叶提取物对一次性全身4 Gy137Cs γ射线照射导致的小鼠造血系统辐射损伤的防护作用。方法 (1)体外实验。采用1,1-二苯基-2-苦基肼(DPPH)和2,2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基体外清除实验检测黄杞叶提取物的体外抗氧化能力。(2)体内实验。①存活实验。将40只无特定病原体(SPF)级雄性C57BL/6小鼠按照区组随机法平均分为4组:照射组、照射+黄杞叶提取物低剂量(20 mg/kg)组、照射+黄杞叶提取物中剂量(40 mg/kg)组、照射+黄杞叶提取物高剂量(80 mg/kg)组, 4组均进行全身一次性7.2 Gy(致死剂量)137Cs γ射线照射, 记录照射后30 d小鼠的存活情况。②造血系统辐射损伤防护实验。将60只SPF级雄性C57BL/6小鼠按照区组随机法平均分为6组:对照组、照射组、黄杞叶提取物高剂量组(80 mg/kg)、照射+黄杞叶提取物低剂量(20 mg/kg)组、照射+黄杞叶提取物中剂量(40 mg/kg)组、照射+黄杞叶提取物高剂量(80 mg/kg)组, 其中对照组和黄杞叶提取物高剂量组不照射, 其余4组均进行一次性全身4 Gy137Cs γ射线照射。照射后第7天处死小鼠, 取外周血进行血常规检测, 取单侧股骨骨髓细胞进行骨髓有核细胞计数, 取另一侧股骨骨髓细胞进行骨髓DNA含量检测, 取胸腺和脾脏计算脏器指数, 取肝脏检测还原型谷胱甘肽(GSH)含量。体内实验中各组小鼠均于照射前7 d和照射后6 d连续灌胃给药, 其中对照组和照射组给予0.5%羧甲基纤维素钠, 其余各组给予相应剂量的黄杞叶提取物。组间两两比较采用Studentt检验, 存活率采用Kaplan-Meier生存分析。结果 (1)在体外实验中, 当浓度分别为100 μg/ml和200 μg/ml时, 与Trolox相比, 黄杞叶提取物对DPPH自由基的清除率更高(89.83%对69.37%, 90.94%对68.53%);对ABTS自由基的清除率也更高(94.81%对71.35%, 94.46%对71.93%), 且差异均有统计学意义(t=19.58~33.26, 均P<0.001)。(2)在体�Objective To study the protective effects of Engelhardia roxburghiana Wall.leaf extract on mice hematopoietic system injury caused by disposable systemic 4 Gy 137Csγ-rays irradiation.Methods(1)In vitro experiment.1,1-Diphenyl-2-picrylhydrazyl(DPPH)and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS)free radical scavenging tests were used to detect the antioxidant activity of Engelhardia roxburghiana Wall.leaf extract in vitro.(2)In vivo experiment.①Survival experiment.Forty specific pathogen-free male C57BL/6 mice were divided into four groups following a randomized block design:irradiation group,irradiation+low dose,irradiation+middle dose,and irradiation+high dose groups of Engelhardia roxburghiana Wall.leaf extract(20,40,and 80 mg/kg,respectively).All four groups were irradiated with whole-body 7.2 Gy(lethal dose)137Csγ-rays at one time.The survival condition of mice 30 days after irradiation was recorded.②Radiation protection experiment of the hematopoietic system.Sixty specific pathogen-free male C57BL/6 mice were divided into six groups following a randomized block design:control group,irradiation group,Engelhardia roxburghiana Wall.leaf extract high dose group(80 mg/kg),irradiation+low dose,irradiation+middle dose,and irradiation+high dose groups of Engelhardia roxburghiana Wall.leaf extract(20,40,and 80 mg/kg,respectively).The control group and the Engelhardia roxburghiana Wall.leaf extract high dose group(80 mg/kg)were not irradiated,while the four other groups were irradiated with whole-body 4 Gy 137Csγ-rays at one time.On the 7th day after irradiation,the mice were killed,the peripheral blood was obtained for blood routine detection,and unilateral femoral bone marrow cells were obtained for bone marrow nucleated cells count.The bone marrow cells of the other femur were used for bone marrow DNA content detection,the thymus and spleen were taken to calculate organ index,and liver was sampled to detect the content of reduced glutathione(GSH).In the in vitro experiment,the mice in each gr

关 键 词：辐射防护 造血系统 抗氧化药(中药) 黄杞叶提取物 

分 类 号：R285.5[医药卫生—中药学]