不同剂量包虫病基因工程亚单位疫苗EG95免疫牦牛后的抗体水平测定  

Determination of the Antibody Level in Yaks Vaccinated with Different Doses of Echinococcosis Genetic Engineering Subunit Vaccine EG95

作　　者：李盛琼 杨光友[2] 李徳珍 陈和强 华瑞其 高露 尹杰 袁东波[1] 杨天俊 黄雅琳 魏秋霞 阳爱国[1] 侯巍[1] Li Shengqiong;Yang Guangyou;Li Dezhen;Chen Heqiang;Hua Ruiqi;Gao Lu;Yin Jie;Yuan Dongbo;Yang Tianjun;Huang Yalin;Wei Qiuxia;Yang Aiguo;Hou Wei(Sichuan Center for Animal Disease Prevention and Control,Chengdu,Sichuan 610000,China;College of Veterinary Medicine,Sichuan Agricultural University,Chengdu,Sichuan 610000,China;Daofu Center for Animal Disease Prevention and Control,Daofu,Sichuan 626400,China;Ganzi Center for Animal Disease Prevention and Control,Ganzi,Sichuan 626700,China;Fushun Bureau of Agricultural and Rural Affairs,Fushun,Sichuan 610000,China;Ya'an Aquaculture Development Center,Ya'an,Sichuan 625000,China)

机构地区：[1]四川省动物疫病预防控制中心,四川成都610041 [2]四川农业大学动物医学院,四川成都611130 [3]道孚县动物疫病预防控制中心,四川道孚626400 [4]甘孜州动物疫病预防控制中心,四川甘孜626700 [5]富顺县农业农村局,四川富顺643200 [6]雅安市养殖业发展中心,四川雅安625000

出　　处：《中国动物检疫》2022年第7期108-110,118,共4页China Animal Health Inspection

基　　金：四川省科技计划项目(2020YFN0030)。

摘　　要：为比较和评价不同剂量包虫病基因工程亚单位疫苗EG95免疫牦牛后的抗体差异,随机选择1岁左右的牦牛50头,平均分成A、B、C、D、E 5组,分别按照1、2、3、4、5头份剂量(每头份含EG9550μg)进行一免和二免(间隔28 d);免疫前及二免后15 d采血,应用间接ELISA法检测血清中的特异性抗体。结果显示:二免后A、B、C组的抗体阳性率分别为30%、40%、50%,与E组的90%存在显著性差异(P<0.05),D组阳性率为80%,与E组无显著性差异(P>0.05);A组和B组抗体合格率均为0,C、D、E组的抗体合格率分别为10%、40%、70%,E组抗体合格率明显高于其他组(P<0.05)。试验结果表明,采用EG95基因工程亚单位疫苗按5头份剂量免疫,间隔28 d后二免,对牦牛的免疫效果较为理想。In order to compare and evaluate the difference of antibody level in yaks vaccinated with different doses of echinococcosis genetic engineering subunit vaccine EG95,50 yaks about one-year old were randomly selected and divided into five groups:A,B,C,D and E.Primary and secondary vaccination(with an interval of 28 days)were conducted at the doses of 1,2,3,4 and 5 heads/pieces(each containing 50μg of EG95),respectively;blood was collected prior to vaccination and 15 days after secondary vaccination to detect the specific antibodies in serum by indirect ELISA.The results showed that,after secondary vaccination,the antibody positive rates of group A,B and C were 30%,40%and 50%,respectively,which were significantly different from 90%of group E(P<0.05),and that of group D was 80%,which was not significantly different from group E(P>0.05);those of group A and group B were 0,those of group C,D and E were 10%,40%and 70%,respectively,and that of group E was significantly higher than that in other groups(P<0.05).It was concluded that good immunity could be produced in yaks when genetic engineering subunit vaccine EG95 was used at the dose of 5 heads/pieces and followed by secondary vaccination with an interval of 28 days.

关 键 词：包虫病 基因工程亚单位疫苗EG95 牦牛 抗体 免疫效果 

分 类 号：S858.23[农业科学—临床兽医学]