多沙唑嗪和羟丙哌嗪对映体的HPLC手性柱拆分  

Enantiomerseparation of Doxazosin and Dropropizine by High Performance Liquid Chromatography Chiral Columns

作　　者：覃信铭 陆金富 潘玉 张家昌 沈静茹[1] Qin Xinming;Lu Jinfu;Pan Yu;Zhang Jiachang;Shen Jingru(Key Laboratory of Analytical Chemistry of the State Ethnic Affair Commission,College of Chemistry and Materials Science,South-Central MinZu University,Wuhan 430074,China)

机构地区：[1]中南民族大学化学与材料科学学院分析化学国家民委重点实验室,湖北武汉430074

出　　处：《山东化工》2022年第11期121-124,共4页Shandong Chemical Industry

基　　金：中央高校基本科研业务费专项资金项目(CZY19037,CZY20036)。

摘　　要：将β-环糊精有机骨架材料作为高效液相色谱手性固定相,并用于构建拆分手性药物对映体的反相高效液相色谱(RP-HPLC)体系。以多沙唑嗪和羟丙哌嗪两种手性药物作为拆分对象,系统地从流动相配比、缓冲溶液pH值、缓冲溶液种类和浓度、流动相流速等方面探究了两对手性对映体的拆分条件。最佳条件下,多沙唑嗪两对映体分离度R_(s)可达1.51。此工作建立了一种拆分多沙唑嗪和羟丙哌嗪对映体的HPLC新方法。Theβ-cyclodextrin organic skeleton material was used as the chiral stationary phase for high performance liquid chromatography(HPLC)and used to establish the reversed-phase HPLC system for the separating doxazosin and dropropizine enantiomers.The enantioseparation conditions of doxazosin and dropropizine were systematically explored from the aspects of mobile phase ratio,pH value of buffer solution,type and concentration of buffer solution,and flow rate of mobile phase.Under the optimal conditions,the resolution R_(s) of doxazosin enantiomers reached 1.51.This work established a new HPLC method for the resolution of doxazosin and hydroxypropyl piperazine enantiomers.

关 键 词：对映体拆分 高效液相色谱 β-环糊精有机骨架手性固定相 多沙唑嗪 羟丙哌嗪 

分 类 号：O657.7[理学—分析化学]