基于共有峰率和变异峰率双指标序列法的桔梗样品红外光谱指纹图谱研究  

Establishment of Infrared Fingerprint of Platycodonis Radix Samples Based on Dual-Index Sequence Analytical Method of Common and Variant Peak Ratio

作　　者：丁波 李国强 许曼 陈冉 DING Bo;LI Guoqiang;XU Man;CHEN Ran(Anhui Province Maternity and Child Health Hospital,Hefei,Anhui,China 230001)

机构地区：[1]安徽省妇幼保健院,安徽合肥230001

出　　处：《中国药业》2022年第13期74-78,共5页China Pharmaceuticals

基　　金：2018年安徽省重点培育智库——安徽省中医药发展研究中心项目[2018zk02]。

摘　　要：目的基于共有峰率和变异峰率双指标序列法建立评价不同产地桔梗样品质量的红外光谱指纹图谱。方法采用溴化钾压片法制备桔梗样品,采用傅里叶红外光谱法,结合多维共有峰率和变异峰率双指标序列法,对7个产地的桔梗样品进行产地区分和质量评价。结果7个产地桔梗样品的红外光谱图基本相似,3280,2929,1740,1635,1419,1034,670,525 cm^(-1)波数附近有特征吸收峰出现,1740,1635,1419,1034 cm^(-1)波数附近吸收峰的位置、数目和强度有差异;双指标序列分析结果显示,样品的共有峰率不低于46.2%,变异峰率不高于66.7%。结论产地接近、气候条件与生长环境相似的桔梗间共有峰率较高,而相差较大的桔梗间变异峰率较高。红外光谱法结合多维共有峰率和变异峰率双指标序列法可准确、直观地鉴别桔梗的真伪、产地和品质。Objective To establish an infrared(IR)fingerprint of Platycodonis Radix samples from different produce areas based on the dual-index sequence analytical method of common and variant peak ratio to evaluate their quality.Methods The potassium bromide pellet was used to prepare the Platycodonis Radix samples.The Fourier transform infrared(FTIR),dual-index sequence analytical method of multi-dimensional common and variant peak ratio were used to identify the produce areas of Platycodonis Radix samples from seven produce areas and evaluate their quality.Results The IR spectrograms of Platycodonis Radix samples from seven produce areas were similar.There were characteristic absorption peaks around wavenumbers of 3280,2929,1740,1635,1419,1034,670,525 cm^(-1),and the location,number and intensity of absorption peaks were different around wavenumbers of 1740,1635,1419 and 1034 cm^(-1).The results of dual-index sequence analytical method showed that the common peak ratio of the samples was not less than 46.2%,while the variation peak ratio of the samples was not higher than 66.7%.Conclusion The common peak ratio among Platycodonis Radix with near produce areas,similar climate and growth environment was higher,while the variant peak ratio among Platycodonis Radix with great differences in the above factors was higher.The IR spectrometry combined with dual-index sequence analytical method of multi-dimensional common and variant peak ratio can accurately and intuitively identify the authenticity,produce area and quality of Platycodonis Radix.

关 键 词：桔梗 红外光谱法 指纹图谱 双指标序列法 共有峰率 变异峰率 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学] R286.0