红松胚性愈伤组织增殖的激素配比、糖源类型和增殖周期效应研究  

Effects of Hormone Combinations,Carbon Source Type and Proliferation Cycle on Embryogenic Callus Proliferation of Pinus koraiensis

作　　者：任毓辉 聂帅 彭春雪 杨玲 沈海龙 REN Yuhui;NIE Shuai;PENG Chunxue;YANG Ling;SHEN Hailong(State Key Laboratory of Tree Genetics and Breeding,School of Forestry,Northeast Forestry University,Harbin 150040)

机构地区：[1]东北林业大学林学院,林木遗传育种国家重点实验室,哈尔滨150040

出　　处：《植物研究》2022年第4期704-712,共9页Bulletin of Botanical Research

基　　金：红松优质大径材定向培育关键技术研究(2017YFD0600601)。

摘　　要：为了解决红松(Pinus koraiensis)胚性愈伤组织增殖过程中生长状态差、体胚产量不高等问题,本研究利用红松胚性愈伤组织1-100细胞系为材料,通过调节红松增殖培养基中激素、碳源和增殖培养周期,探索其对红松体胚发生能力的影响及其生理生化特性变化。结果如下:①红松胚性愈伤组织最佳增殖条件激素浓度为1 mg·L^(-1) NAA+0.25 mg·L^(-1)6-BA,最佳碳源为蔗糖,最佳增殖培养周期为14 d,在此条件下获得的体胚产量最多(166个·g^(-1));②在优化增殖培养过程中,储藏物质为红松体胚发生提供了能量来源;激素种类及其浓度、碳源种类、增殖培养周期之间可溶性蛋白质量分数没有显著差异;③红松愈伤组织中CAT活性过高不利于愈伤组织的体胚发生,可能是H_(2)O_(2)等代谢产物积累过多,产生毒害作用。In order to solve the poor growth and low somatic embryo production in the embryogenic callus proliferation of Pinus koraiensis,P.koraiensis embryogenic callus 1-100 cell line was used as material to explore its effect on the somatic embryogenesis and the changes of physiological and biochemical characteristics by adjusting hormone,carbon source and proliferation culture cycle in proliferation medium.The results were as follows:(1)the optimum proliferation condition was 1 mg·L^(-1)NAA+0.25 mg·L^(-1) 6-BA,the best carbon source was sucrose,and the best culture cycle was 14 days.Under the conditions,the largest number of somatic embryos were obtained(166·g^(-1));(2)in the process of optimizing proliferation culture,the stored substances provided energy for somatic embryogenesis;There was no significant difference in soluble protein mass fraction between hormone types and their concentrations,carbon source types and proliferation culture cycles;(3)high CAT activity in callus was harmful to somatic embryogenesis,which might be due to excessive accumulation of toxic metabolites such as H_(2)O_(2).

关 键 词：红松 胚性愈伤组织 体细胞胚胎发生 增殖培养基 

分 类 号：S722.8[农业科学—林木遗传育种]