槲皮素脂质纳米粒冻干粉末体外稳定性及肝癌细胞毒性研究  

Study on the Stability of Quercetin Lipid Nanoparticles Freeze-dried Powder and its Cytotoxicity to Liver Tumor in Vitro

作　　者：苗方[1] 马兰 MIAO Fang;MA Lan(Heze Medical College,Heze Shandong 274000,China.)

机构地区：[1]菏泽医学专科学校,山东菏泽274000

出　　处：《药品评价》2022年第8期449-453,共5页Drug Evaluation

基　　金：山东省医药卫生科技发展计划项目(202013021494)。

摘　　要：目的:研究槲皮素脂质纳米粒冻干粉末的体外稳定性及其体外对肝癌细胞毒性。方法:使用热熔乳化超声分散法制备槲皮素脂质纳米粒,采用紫外分光光度法测定槲皮素吸光度并计算载药量和包封率,激光粒度测定仪测定脂质纳米粒的粒径和电位,透析法测定体外释放行为,四甲基偶氮唑蓝比色法测定其体外对HepG2细胞毒性作用。结果:制备的槲皮素脂质纳米粒冻干粉末放置60 d内稳定性较好,粒径、电位、载药量和包封率未发生明显变化;槲皮素体外释放在48 h内呈现缓慢释放效果,且释放较完全;细胞毒性试验表明,保存60 d内的槲皮素脂质纳米粒对HepG2细胞毒性明显强于槲皮素原料药。结论:保存60 d内的槲皮素脂质纳米粒冻干粉末稳定性良好,具有明显地抑制HepG2细胞的作用。Objective:To study the in vitro stability of quercetin lipid nanoparticles freeze-dried powder and its in vitro toxicity to hepatocellular carcinoma.Methods:Quercetin lipid nanoparticles were prepared by hot melt emulsification and ultrasonic dispersion method.The absorbance of quercetin was measured by UV spectrophotometry and the drug loading and encapsulation rate were calculated.The particle size and potential of quercetin lipid nanoparticles were measured by laser particle size analyzer.In vitro release behavior was determined by dialysis,and toxicity of HepG2 cells was determined by tetramethyl azazole blue colorimetry.Results:The freeze-dried quercetin lipid nanoparticles prepared by the optimal formulation had good stability after 60 days of storage,and the particle size,potential,drug loading and encapsulation rate did not change significantly.Quercetin was released slowly and completely within 48 h.Cytotoxicity test showed that the cytotoxicity of stored for 60 daysquercetin lipid nanoparticles to HepG2 cells was significantly superior to quercetin bulk drug.Conclusion:The freeze-dried quercetin lipid nanoparticles stored for 60 days have good stability and can significantly inhibit HepG2 cells.

关 键 词：槲皮素 HEPG2细胞 肝肿瘤 脂质纳米粒 稳定性 细胞毒性 

分 类 号：R285[医药卫生—中药学]