IL-36在哮喘患儿血清中的水平及临床意义  

The levels and clinical significance of serum IL-36 in children with bronchial asthma

作　　者：肖夏夏 韩春芳[1] 于海英[1] 李明磊[1] 郑加香 孙中厚 XIAO Xiaxia;HAN Chunfang;YU Haiying;LI Minglei;ZHENG Jiaxiang(Pediatrics Department,Weifang People’s Hospital,Weifang 261041,China)

机构地区：[1]潍坊市人民医院儿科,山东潍坊261041 [2]不详

出　　处：《潍坊医学院学报》2022年第1期64-67,共4页Acta Academiae Medicinae Weifang

基　　金：潍坊市卫生健康委科研项目(项目编号:wfwsjk-2019-193)。

摘　　要：目的 通过检测支气管哮喘患儿血清中IL-36水平,及其与嗜酸性粒细胞(EOS)和呼出气一氧化氮(FeNO)及呼气峰流速(PEF)的相关性,探讨IL-36在哮喘发病中的作用及临床意义。方法 选取2019年11月~2020年10月在潍坊市人民医院小儿内科门诊就诊及病房住院哮喘患儿65例,其中急性发作组患儿30例,临床缓解组患儿35例。选择同时期同年龄段于我院进行常规体检的健康儿童25例作为对照组。采用酶联免疫吸附法(ELISA)测定3组研究对象血清IL-36水平;采用细胞分析仪常规方法检测外周血EOS计数;采用一氧化氮测定仪和肺功能仪分别检测各组儿童FeNO和PEF,对结果进行统计学分析。结果 (1)急性发作组患儿血清IL-36水平(53.76±10.60)ng/L显著高于临床缓解组(28.53±7.02)ng/L和对照组(24.25±6.48)ng/L(P均<0.01);临床缓解组患儿IL-36水平高于对照组(P<0.05)。(2)急性发作组患儿EOS计数(1.28±0.14)×10^(9)/L显著高于临床缓解组(0.77±0.17)×10^(9)/L和对照组(0.23±0.12)×10^(9)/L(P均<0.01);临床缓解组患儿EOS计数显著高于对照组(P<0.01)。(3)急性发作组患儿FeNO(44.30±7.45)ppb显著高于临床缓解组(28.14±5.54)ppb和对照组(10.32±6.45)ppb(P均<0.01);临床缓解组患儿FeNO显著高于对照组(P<0.01)。(4)急性发作组患儿PEF(75.40±8.41)%明显低于临床缓解组(87.94±6.70)%和对照组(93.04±5.85)%(P均<0.01);临床缓解组患儿PEF显著低于对照组(P<0.01)。(5)哮喘患儿血清IL-36水平与EOS、FeNO均呈正相关(r=0.807,P<0.01;r=0.833,P<0.01);哮喘患儿血清IL-36水平与PEF呈负相关(r=-0.701,P<0.01)。结论 IL-36作为促炎因子参与儿童哮喘的发病过程,临床检测IL-36血清水平对哮喘诊断及管理具有指导作用。Objective To investigate the clinical significance of interleukin-36(IL-36) in children with bronchial asthma by detecting the serum levels of IL-36,eosinophil(EOS),fractional exhaled nitric oxide(FeNO) and peak expiratory flow(PEF),and analyzing the correlation among them.Methods Sixty-five children with asthma were enrolled from November 2019 to October 2010 in Weifang People’s Hospital, among them, 30 cases were in acute exacerbation, 35 cases were in remission.Twenty-five healthy children who underwent routine physical examination in our hospital at the same time and the same age served as controls.The serum levels of IL-36 were detected by enzyme-linked immunosorbent assay(ELISA),the EOS count in peripheral blood was measured by hematology analyzer, the FeNO and the PEF were measured by the NO analyzer and pulmonary function instrument, and the statistical analysis was performed.Results(1)The serum levels of IL-36 in acute exacerbation group(53.76±10.60)ng/L were significantly higher than those in remission group(28.53±7.02)ng/L and control group(24.25±6.48)ng/L(P all<0.01);and the serum levels of IL-36 in remission group were higher than those in control group(P<0.05).(2)The EOS counts in acute exacerbation group(1.28±0.14)×10^(9)/L were significantly higher than those in remission group(0.77±0.17)×10^(9)/L and control group(0.23±0.12)×10^(9)/L(P all<0.01);and the EOS counts in remission group significantly were higher than those in health control group(P<0.01).(3)The FeNO in acute exacerbation group(44.30±7.45)ppb was significantly higher than that in remission group(28.14±5.54)ppb and control group(10.32±6.45)ppb(P all<0.01),the FeNO in remission group was significantly higher than that in control group(P<0.01).(4)The PEF in acute exacerbation group(75.40±8.41)% was obviously lower than it in remission group(87.94±6.70)% and control group(93.04±5.85)%(P all<0.01);and the PEF in remission group obviously lower than it in control group(P<0.01).(5)The serum levels of IL-36 were positively co

关 键 词：白细胞介素-36 嗜酸性粒细胞 呼出气一氧化氮 呼气峰流速 儿童哮喘 

分 类 号：R725.6[医药卫生—儿科]