OsbZIP60-mediated unfolded protein response regulates grain chalkiness in rice  

作　　者：Weiping Yang Pengkun Xu Juncheng Zhang Shuo Zhang Zhenwei Li Ke Yang Xinyuan Chang Yibo Li 

机构地区：[1]aNational Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research(Wuhan),Huazhong Agricultural University,Wuhan,Hubei 430070,China [2]Hubei Hongshan Laboratory,Wuhan,Hubei 430070,China

出　　处：《Journal of Genetics and Genomics》2022年第5期414-426,共13页遗传学报（英文版）

基　　金：supported by grants from the National Key Research and Development Program of China (2021YFF1000202);the National Natural Science Foundation of China (31771368, 31821005,31701397);Ten-thousand Talents Programs of China;Fundamental Research Funds for the Central Universities (2662020SKPY006);Wuhan Applied Foundational Frontier Project (2020020601012257);Hubei Hongshan Laboratory (2021hszd005),China

摘　　要：Grain chalkiness,an undesirable trait caused by complex factors,has great negative impacts on the quality and economic value of rice.However,little is known about the regulatory mechanism of grain chalkiness,particularly the effect of endoplasmic reticulum(ER)stress.Here,a genome-wide association study(GWAS)reveals that the transcription factor Osb ZIP60 is a vital regulator of rice grain chalkiness.Genetic analysis demonstrates that knockout of Osb ZIP60 results in extremely high grain chalkiness and aberrant structure of storage substances.Notably,the expression of unfolded protein response(UPR)genes,such as OsbZIP50,Os Bi P1,Os Bi P2 and Os Bi P3,is up-regulated in the endosperm cells of osbzip60,and overexpression of all these UPR genes causes various degrees of chalkiness.Furthermore,Osb ZIP60 is found to activate the expression of key genes related to grain chalkiness,such as GPA3,FSE1,FLO7,Chalk5,Os NF-YB1,and Os PK2,whose expression is significantly suppressed in osbzip60 and overexpression lines of Osb ZIP50,Os Bi P1,Os Bi P2,and Os Bi P3.Our study provides novel insights into the function of Osb ZIP60 and the role of the UPR pathway in the formation of grain chalkiness in rice.

关 键 词：RICE Grain chalkiness GWAS OsbZIP60 OsbZIP50 UPR 

分 类 号：S511[农业科学—作物学]