OsLTP47 may function in a lipid transfer relay essential for pollen wall development in rice  

作　　者：Libin Chen Chonghui Ji Degui Zhou Xin Gou Jianian Tang Yongjie Jiang Jingluan Han Yao-Guang Liu Letian Chen Yongyao Xie 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,College of Life Sciences,South China Agricultural University,Guangzhou,Guangdong 510642,China [2]Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou,Guangdong 510642,China [3]Guangdong Key Laboratory of New Technology in Rice Breeding,Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong 510640,China [4]Present address:Vector-borne Virus Research Center,Fujian Province Key Laboratory of Plant Virology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China.

出　　处：《Journal of Genetics and Genomics》2022年第5期481-491,共11页遗传学报（英文版）

基　　金：supported by grants from the Guangdong Natural Science Funds for Distinguished Young Scholars (2021B1515020089);the National Natural Science Foundation of China (32030080);the Major Program of Guangdong Basic and Applied Research(2019B030302006)

摘　　要：In plants,lipid transfer proteins(LTPs)transport pollen wall constituents from the tapetum to the exine,a process essential for pollen wall development.However,the functional cooperation of different LTPs in pollen wall development is not well understood.In this study,we have identified and characterized a grassspecific LTP gene,Os LTP47,an important regulator of pollen wall formation in rice(Oryza sativa).Os LTP47 encodes a membrane-localized LTP and in vitro lipid-binding assays confirms that Os LTP47 has lipidbinding activity.Dysfunction of Os LTP47 causes disordered lipid metabolism and defective pollen walls,leading to male sterility.Yeast two-hybrid and pull-down assays reveal that Os LTP47 physically interacts with another LTP,Os C6.These findings suggest that the plasma membrane-localized Os LTP47 may function as a mediator in a lipid transfer relay through association with cytosolic and/or locular Os C6 for pollen wall development and that various LTPs may function in a coordinated manner to transport lipid molecules during pollen wall development.

关 键 词：RICE Pollen wall Lipid transfer protein Male sterility 

分 类 号：S511[农业科学—作物学]