Maternal-derived antibodies hinder the antibody response to H9N2 AIV inactivated vaccine in the field  

作　　者：Xue Pan Xin Su Pingyun Ding Jinhua Zhao Hongrui Cui Dawei Yan Qiaoyang Teng Xuesong Li Nancy Beerens Haitao Zhang Qinfang Liu Mart C.M.de Jong Zejun Li 

机构地区：[1]Shanghai Veterinary Research Institute,Chinese Academy of Agriculture Sciences,Postal Address:Office Room D301,518 Ziyue Road,Minhang District,Shanghai 200241,China [2]Quantitative Veterinary Epidemiology,Animal Sciences Group,Wageningen University&Research,Postal Address:Postbus 338,6700AH Wageningen,the Netherlands [3]Biotechnology Research laboratory,Jiangsu Lihua Animal Husbandry Co.Ltd.,Changzhou,China [4]Wageningen Bioveterinary Research,Wageningen University&Research,Lelystad,the Netherlands

出　　处：《Animal Diseases》2022年第2期109-117,共9页动物疾病（英文）

基　　金：This study was supported by grants from the National Key Research and Development Plan(Nos.2016YFD0500204 and 2017YFD0500800);National Natural Science Foundation of China(Nos.31772753,31572543,31700136 and 31702237);Shanghai Municipal Natural Science Foundation(No.17ZR1437400);the Project of the Shanghai Science and Technology Commission(No.17391901700).

摘　　要：The H9N2 subtype avian influenza virus(AIV)inactivated vaccine has been used extensively in poultry farms,but it often fails to stimulate a sufficiently high immune response in poultry in the field,although it works well in laboratory experiments;hence,the virus still causes economic damage every year and poses a potential threat to public health.Based on surveillance data collected in the field,we found that broilers with high levels of maternal-derived antibodies(MDAs)against H9N2 virus did not produce high levels of antibodies after vaccination with a commercial H9N2 inactivated vaccine.In contrast,specific pathogen-free(SPF)chickens without MDAs responded efficiently to that vaccination.When MDAs were mimicked by administering passively transferred antibodies(PTAs)into SPF chickens in the laboratory,similar results were observed:H9N2-specific PTAs inhibited humoral immunity against the H9N2 inactivated vaccine,suggesting that H9N2-specific MDAs might hinder the generation of antibodies when H9N2 inactivated vaccine was used.After challenge with homologous H9N2 virus,the virus was detected in oropharyngeal swabs of the vaccinated and unvaccinated chickens with PTAs but not in the vaccinated chickens without PTAs,indicating that H9N2-specific MDAs were indeed one of the reasons for H9N2 inactivated vaccine failure in the field.When different titers of PTAs were used to mimic MDAs in SPF chickens,high(HI=12 log2)and medium(HI=log 9 log2)titers of PTAs reduced the generation of H9N2-specific antibodies after the first vaccination,but a booster dose would induce a high and faster humoral immune response even of PTA interference.This study strongly suggested that high or medium titers of MDAs might explain H9N2 inactivated vaccine failure in the field.

关 键 词：Maternal-derived antibodies(MDAs) Passively transferred antibodies(PTAs) Humoral immune response Vaccination failure H9N2 avian influenza virus(AIV) 

分 类 号：S85[农业科学—兽医学]