嗜水气单胞菌外膜蛋白缺失株逆境响应功能评价  

Evaluation of Aeromonas hydrophila outer membrane protein deletion strains in response to environmental stresses

作　　者：李泽琦 汪玉倩 李小艳 林向民 袁军 LI Zeqi;WANG Yuqian;LI Xiaoyan;LIN Xiangmin;YUAN Jun(Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring,College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学生命科学学院,福建省农业生态过程与安全监控重点实验室,福州350002

出　　处：《浙江大学学报（农业与生命科学版）》2022年第3期303-309,共7页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：福建省自然科学基金重点项目(2020J02023)。

摘　　要：为系统评价外膜蛋白在环境胁迫条件下的生物学功能,以嗜水气单胞菌(Aeromonas hydrophila)ATCC 7966为研究对象,选取33株外膜蛋白缺失菌株,对其贴太紧在渗透压、金属离子、H_(2)O_(2)及pH等胁迫条件下的生长状态进行测定。结果发现:编码TonB家族蛋白基因AHA_0461、AHA_4275被敲除的菌株在渗透压胁迫条件下较野生型菌株生长更好;编码未知功能蛋白基因AHA_2282被敲除的菌株在金属离子胁迫条件下生长较弱,而ΔAHA_0904菌株在渗透压胁迫条件下生长状况较好;编码长链脂肪酸转运蛋白基因AHA_2145被敲除的菌株在H_(2)O_(2)和金属离子胁迫条件下生长状态较弱;而编码OmpA家族蛋白基因AHA_1279、AHA_1281和分泌素家族蛋白基因pilQ、AHA_0569被敲除的菌株在H_(2)O_(2)胁迫条件下的生长均弱于野生型菌株。提示这些蛋白可能在细菌响应环境胁迫方面起重要作用。本研究结果有望为今后全面地了解嗜水气单胞菌外膜蛋白响应不同环境胁迫的机制提供思路,为该病原菌的防治提供可能的靶点。In order to evaluate systematically the biological functions of outer membrane proteins(OMPs)under environmental stresses,we selected Aeromonas hydrophila ATCC 7966 as the research object,and constructed 33 OMP deletion strains to determine their growth status under the stress conditions of osmotic pressure,metal ions,H_(2)O_(2) and pH.The results showed that the AHA_0461 and AHA_4275(encoding TonB protein family)knockout strains grew better than the wild type strain under the osmotic pressure stress.The AHA_2282(encoding an unknown functional protein)knockout strain grew weakly under the metal ion stresses,whileΔAHA_0904 strain grew better under the osmotic pressure stress.The AHA_2145(encoding long-chain fatty acid transporter)knockout strain grew weakly under the H_(2)O_(2) and metal ion stresses.The growth of AHA_1279,AHA_1281(encoding OmpA family protein)and pilQ,AHA_0569(encoding secretin family protein)knockout strains was weaker than the wild type strain under the H_(2)O_(2) stress.It was suggested that these OMPs may play important roles in response to environmental stresses.These results may highlight a comprehensive understanding of the physiological functions of bacterial OMPs under different environmental stresses in the future,and provide possible targets for the prevention and control of this pathogen.

关 键 词：嗜水气单胞菌 外膜蛋白 基因缺失 逆境响应 

分 类 号：Q93-3[生物学—微生物学]