瘤胃液、血浆及牛奶中奇链支链脂肪酸含量与其前体物含量的相关性  

Correlation between Content of Odd-and Branched-Chain Fatty Acids in Rumen Fluid,Plasma and Milk and Their Precursors Content

作　　者：孙玲玲[1] 高胜涛 马露[1,2] 沈旖帆 温万 卜登攀[1,2] SUN Lingling;GAO Shengtao;MA Lu;SHEN Yifan;WEN Wan;BU Dengpan(State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academia of Agricultural Sciences,Beijing 100193,China;Chinese Academy of Agricultural Sciences and World Agroforestry Center Joint Lab on Agroforestry and Sustainable Animal Husbandry,Beijing 100193,China;Animal Husbandry Extension Station,Yinchuan 750001,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京100193 [2]中国农业科学院与世界农用林业中心农用林业与可持续畜牧业联合实验室,北京100193 [3]宁夏畜牧工作站,银川750001

出　　处：《动物营养学报》2022年第6期3645-3656,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：宁夏回族自治区重点研发计划项目(2021BEF02018);中央级公益性科研院所基本科研业务费专项(2020-YWF-YB-07);中国农业科学院科技创新工程(ASTIP-IAS07-1);奶牛产业技术体系北京市创新团队(BAIC06-2021)。

摘　　要：本试验旨在探究瘤胃中合成奇链支链脂肪酸(OBCFA)的前体物与瘤胃液、血浆及牛奶中OBCFA含量的相关性。选择体重、胎次、泌乳日龄和产奶量相近的健康荷斯坦奶牛27头,所有奶牛饲养在同一圈舍中饲喂相同饲粮4周,于最后3 d采集奶牛的瘤胃液、血液及牛奶样品,分别测定瘤胃液中支链氨基酸(BCAA)、挥发性脂肪酸(VFA)及OBCFA含量以及血浆、牛奶中OBCFA含量。结果表明:1)瘤胃液中丙酸和戊酸含量与奇链脂肪酸含量的相关性较弱,瘤胃液中支链VFA和BCAA均能在瘤胃液中找到与之具有显著(P<0.05)或极显著(P<0.01)相关关系的OBCFA,以上相关关系多属于中等强度相关。2)瘤胃液中丙酸和戊酸含量与血浆中十七烷酸(n-17∶0)含量呈显著正相关(P<0.05),瘤胃液中支链VFA和BCAA均能在血浆中找到与之具有显著(P<0.05)或极显著(P<0.01)相关关系的OBCFA,以上相关关系多属于弱相关或中等强度相关。3)瘤胃液中丙酸含量与牛奶中奇链脂肪酸含量无显著相关性(P>0.10),而瘤胃液中戊酸含量与牛奶中n-17∶0含量趋于显著正相关(P<0.10)。瘤胃液中支链VFA和BCAA与牛奶中OBCFA存在较弱的相关关系。4)瘤胃液与牛奶中的OBCFA含量具有中等强度的相关关系。由此可见,改变瘤胃中合成OBCFA前体物的含量是一种提高牛奶中OBCFA含量的潜在营养调控策略。This experiment aimed to explore the correlation between the synthetic precursors of odd-and branched-chain fatty acids(OBCFA)in rumen and the content of OBCFA in rumen fluid,plasma and milk.Twenty-seven healthy Holstein dairy cows with similar body weight,parity,lactation age and milk yield were selected,all cows were kept in the same house and fed the same diet for 4 weeks. The rumen fluid,blood and milk samples of the cows were collected on the last 3 days,and the contents of branched-chain amino acids(BCAA),volatile fatty acids(VFA)and OBCFA in rumen fluid and the content of OBCFA in plasma and milk were determined. The results showed as follows:1)the correlation between propionic acid and valeric acid contents and odd-chain fatty acids content in rumen fluid was weak,both branched-chain VFA and BCAA in rumen fluid could find the OBCFA in rumen fluid with significant(P<0.05)or extremely significant(P<0.01)correlation in rumen fluid,and the above correlations were mostly medium-strength correlations. 2)The contents pf propionic acid and valeric acid in rumen fluid were significantly positively correlated with plasma nheptadecanoic acid(n-17∶0)content,both branched chain VFA and BCAA in rumen fluid could find the OBCFA in plasma with significant(P<0.05)or extremely significant(P<0.01)correlation in the plasma,and the above correlations were mostly weak or moderately strong. 3)The content of propionic acid in rumen fluid and the content of odd-chain fatty acids in milk were not significant correlation(P>0.10),and the content of valeric acid in rumen fluid and the content of n-17∶0 in milk tended to be significantly positively correlated(P<0.10). There was a weak correlation between the contents of branched-chain VFA and BCAA in rumen fluid and the content of OBCFA in milk. 4)There was a moderately strong correlation of content of OBCFA in rumen fluid and milk. In conclusion,changing the content of synthetic OBCFA precursors in rumen is a potential nutritional regulation strategy to increase the content of OB

关 键 词：奶牛 奇链支链脂肪酸 支链氨基酸 挥发性脂肪酸 相关性 

分 类 号：S823[农业科学—畜牧学]