新型小麦种衣剂JML对迟播冬小麦苗期生长和碳、氮代谢相关基因表达的影响  

Response of the late sowing winter wheat seedling stage and carbon and nitrogen metabolism related gene expression to the new wheat seed coating agent JML

作　　者：张小祥[1] 肖宁[1] 张新钵 沈卫华[3] 季红娟[1] 李育红[1] 潘存红[1] 吉春明[1] 吴政[1] 吴云雨[1] 蔡跃 刘建菊 余玲[1] 陈梓春 时薇 张秀琴[1] 刘广青[1] 周长海[1] 姚友礼[2] 黄年生[1] 李爱宏[1] ZHANG Xiao-xiang;XIAO Ning;ZHANG Xin-bo;SHEN Wei-hua;JI Hong-juan;LI Yu-hong;PAN Cun-hong;JI Chun-ming;WU Zheng;WU Yun-yu;CAI Yue;LIU Jian-ju;YU Ling;CHEN Zi-chun;SHI Wei;ZHANG Xiu-qin;LIU Guang-qing;ZHOU Chang-hai;YAO You-li;HUANG Nian-sheng;LI Ai-hong(Institute of Agricultural Sciences of the Lixiahe District in Jiangsu Province/Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou 225007,China;College of Agriculture,Yangzhou University/Jiangsu Provincial Key Laboratory of Crop Cultivation and Physiology,Yangzhou 225009,China;Jiangsu Dazhong Farm Group Co.,Ltd.,Dafeng 224135,China)

机构地区：[1]江苏里下河地区农业科学研究所/江苏省粮食作物现代产业技术协同创新中心,江苏扬州225007 [2]扬州大学农学院/江苏省作物栽培生理重点实验室,江苏扬州225009 [3]江苏大中农场集团有限公司,江苏大丰224135

出　　处：《江苏农业学报》2022年第3期625-634,共10页Jiangsu Journal of Agricultural Sciences

基　　金：国家现代农业产业技术体系建设专项(CARS-01-60);江苏省重点研发计划项目(BE2018362);江苏省农业科技自主创新基金项目[CX(18)1002,CX(19)3060];扬州市社会发展项目(YZ2019050)。

摘　　要：为明确新型小麦种衣剂(JML)对迟播冬小麦幼苗生长及碳、氮代谢相关基因表达的影响,以扬麦23、扬麦24为试验材料,分析JML包衣处理后迟播冬小麦在种子活力、根系特征、干物质积累及碳、氮代谢相关基因表达等方面的变化特征。结果表明,JML处理后,扬麦23、扬麦24的发芽势和发芽率显著提高。茎叶与根器官中干物质积累增加,显著提高了植株氮积累量。根长、根表面积、根体积及根直径增加,与对照相比差异显著。JML处理提高了氮代谢中氮素吸收利用关键基因TaNRT1.1、TaNR、TaNIR、TaGS1、TaGS2等在幼苗植株中的表达水平,差异显著,但显著抑制了铵态氮转运蛋白基因TaAMT1.2的表达水平,促进了碳代谢中与光合作用密切相关的TaClpP、TaRBC、TaPSB基因的表达。因此,JML处理可以显著改善迟播冬小麦的种子活力,提高根系综合素质,增加苗期硝态氮的吸收转运、同化水平和叶片的碳同化能力,最终增加苗期植株干物质积累。In order to clarify the effects of the new wheat seed coating agent(JML)on the growth of late sowing winter wheat seedlings and the expression of carbon and nitrogen metabolism-related genes,Yangmai23 and Yangmai24 were used as test materials to analyze the characteristics of seed vigor,root characteristics,dry matter accumulation,and expression of genes related to carbon and nitrogen metabolism of the late sowing winter wheat after JML treatment.The results showed that the germination vigor and germination rate of Yangmai23 and Yangmai24 were significantly improved after JML treatment.Dry matter accumulation in stems,leaves and root organs increased,which significantly increased plant nitrogen accumulation.Root length,root surface area,root volume and root diameter increased,which were significantly different compared with the control.JML treatment increased the expression levels of TaNRT1.1,TaNR,TaNIR,TaGS1,TaGS2 and other key genes for nitrogen obsorption and utilization in seedlings plants during nitrogen metabolism,and the difference was significant(P<0.05).However,the expression of the ammonium nitrogen transporter gene TaAMT1.2 was significantly inhibited,and the expression of TaClpP,TaRBC and TaPSB genes closely related to photosynthesis in carbon metabolism was promoted.Therefore,JML treatment can significantly improve the seed vigor of late-sown winter wheat,improve the overall quality of the root system,increase the level of nitrate nitrogen absorption and assimilation at the seedling stage and the carbon assimilation capacity of the leaves,and ultimately increase the dry matter accumulation of the plant at the seedling stage.

关 键 词：冬小麦 幼苗 种子包衣剂 氮、碳代谢 基因表达 

分 类 号：Q786[生物学—分子生物学]