生物竞争抑制作用对渤海J油田微生物降解驱油聚合物功能的影响  

Impact of bio-competitive exclusion on the degradation of polymers for oil displacement by endogenous microorganisms in Bohai J-oilfield

作　　者：张世仑 王大威[1,2] 尹榆钧 靖波 ZHANG Shilun;WANG Dawei;YIN Yujun;JING Bo(State Key Laboratory of Offshore Oil Exploitation,Beijing 100028,China;China National Offshore Oil Corporation(CNOOC)Research Institute,Beijing 100028,China;Key Laboratory of Molecular Microbiology and Technology,Ministry of Education,College of Life Sciences,Nankai University,Tianjin 300071,China)

机构地区：[1]海洋石油高效开发国家重点实验室,北京100028 [2]中海油研究总院有限责任公司,北京100028 [3]南开大学生命科学学院,分子微生物学与技术教育部重点实验室,天津300071

出　　处：《微生物学报》2022年第6期2289-2298,共10页Acta Microbiologica Sinica

基　　金：国家科技重大专项(2016ZX05025003)。

摘　　要：【目的】揭示可降解驱油用聚合物的油藏内源微生物群落组成,分析生物竞争抑制作用(bio-competitive exclusion,BCX)对微生物聚合物降解功能的影响。【方法】通过室内培养实验,观察BCX对驱油用聚合物黏度的影响,随后借助高通量测序技术分析渤海J油田中与聚合物降解相关的微生物菌种,并探寻样本中丰度较高的聚合物降解功能基因─酰胺酶、加氧酶、硫化氢生成酶基因。之后,比对测序结果,采用实时荧光定量法验证上述功能基因在样本之间的含量差异,最后进一步注释携带上述功能基因的微生物群落组成。【结果】BCX可有效地延缓驱油聚合物黏度的损失。油田中与聚合物降解相关的微生物有Acetomicrobium、Tepidiphilus、Thermoanaerobacter、Fervidobacterium、Ralstonia、Halomonas、Roseovarius、Deferribacteraceae和Comamonadaceae等9类菌种。高通量测序分析得到样本中BCX可显著下调丰度的聚合物降解功能基因共计有7种,其中酰胺酶基因ansB、加氧酶基因ssuD在样本之间的含量经定量验证,发现受到了BCX的抑制,这与测序结果相一致。群落组成注释表明,BCX对渤海J油田聚合物高效降解菌─代尔夫特菌(Delftia)存在明显的抑制作用。【结论】揭示了油田中可降解驱油用聚合物的内源微生物群落组成,发现BCX对微生物降解聚合物的抑制效用,证实BCX有助于解决海上油田聚合物驱稳粘问题。[Objective] This study aimed to reveal the composition of endogenous microbial community degrading the polymers for oil displacement in the reservoir and analyze the influence of bio-competitive exclusion on the microbial degradation of polymers.[Methods] Through indoor culture experiments,we observed the influence of bio-competitive exclusion on the viscosity of the polymers,and used high-throughput sequencing technology to analyze the microbial strains related to polymer degradation in Bohai J-oilfield.We then mined the genes(encoding amidase,oxygenase,hydrogen sulfide-generating enzyme) with high abundance and involved in polymer degradation in the samples.After that,we employed the real-time fluorescence PCR to compare the abundance of the above-mentioned functional genes between samples,and finally annotated the microbial taxa carrying the above-mentioned functional genes.[Results] Bio-competitive exclusion mitigated the viscosity loss of the polymers for oil displacement.Nine taxa of microorganisms related to the degradation of polymers were identified,including Acetomicrobium,Tepidiphilus,Thermoanaerobacter,Fervidobacterium,Ralstonia,Halomonas,Roseovarius,Deferribacteraceae,and Comamonadaceae.High-throughput sequencing revealed that bio-competitive exclusion significantly down-regulated the abundance of 7 genes involved in the polymer degradation in the samples.The measurement of abundance in samples showed that the abundance of the amidase gene ansB and the oxygenase gene ssuD was down-regulated by bio-competitive exclusion,which was consistent with the sequencing results.The community composition annotation indicated that bio-competitive exclusion significantly inhibited Delftia,a highly efficient bacterial taxa for polymer degradation in Bohai J-oilfield.[Conclusion] We revealed the endogenous microbial taxa related to the degradation of polymers for oil displacement in the oilfield and discovered the inhibitory effect of bio-competitive exclusion on the degradation,confirming that bio-competitive exclu

关 键 词：群落组成 生物竞争抑制 黏度 高通量测序 聚合物驱 

分 类 号：TE53[石油与天然气工程—油气田开发工程]