菜芙蓉多糖对秀丽隐杆线虫抗氧化作用的研究  

Study on antioxidantactivity of Abelmoschus manihot(L.)medic polysaccharide on Caenorhabditis elegans

作　　者：杜星芳 贾艳丽 韩紫薇 仇燕[1] DU Xingfang;JIA Yanli;HAN Ziwei;QIU Yan(School of Food Science and Biology,Hebei University of Science and Technology,Shijiazhuang,Hebei 050018,China)

机构地区：[1]河北科技大学食品与生物学院,河北石家庄050018

出　　处：《河北科技大学学报》2022年第3期308-318,共11页Journal of Hebei University of Science and Technology

基　　金：河北省重点研发计划项目(22322801D);京津冀三地联合攻关项目(19YFSLQY00100)。

摘　　要：为进一步研究菜芙蓉多糖(AMP)的体内生物学作用,系统探究其抗氧化机理,以秀丽隐杆线虫(Caenorhabditis elegans)为模型,分析了正常生长条件及氧化应激条件下,空白对照组(0 mg/mL)、低剂量组(25 mg/mL)、中剂量组(50 mg/mL)、高剂量组(100 mg/mL)AMP对线虫生理指标、生化指标及细胞凋亡的影响。结果表明,正常生长条件下AMP可延长线虫寿命、降低生殖能力、提高运动能力,降低线虫体内MDA和ROS含量、提高SOD,CAT和GSH-Px的酶活性。在200μmol/L胡桃醌氧化应激条件下,AMP可提高线虫存活率,并能有效清除体内ROS、降低MDA含量,提高线虫体内SOD,CAT和GSH-Px的酶活性,抑制细胞凋亡。AMP可通过提高线虫体内抗氧化防御系统酶活性及清除自由基提高氧化应激抵抗力,因此AMP有潜力成为一种新的抗氧化天然多糖。In order to study the biological roles of Abelmoschus manihot(L.)medic polysaccharide(AMP)in vivo and the underlying mechanism,C.elegans was used as a model to analyse the influence of ck(0 mg/mL),low dose group(25 mg/mL),medium dose group(50 mg/mL)and high dose group(100 mg/mL)AMP on physiological indexes,biochemical indexes and apoptosis of nematodes.The results show that AMP could prolong the life span of C.elegans,reduce the reproductive capacity,improve the motor capacity,lower the content of MDA and ROS,and increase the activities of SOD,CAT and GSH-Px under normal growth conditions.Under the condition of 200μmol/L juglone oxidative stress,AMP can improve the survival rate of nematodes,effectively eliminate ROS,reduce the content of MDA,increase the activities of SOD,CAT and GSH-Px and inhibit cell apoptosis.AMP can enhance the resistance of C.elegans to oxidative stress by increasing the enzyme activities of antioxidant defense system and scavenging free radicals.AMP has the potential to be a new kind of natural polysaccharide with antioxidant activity.

关 键 词：多糖生物化学 菜芙蓉多糖 秀丽隐杆线虫 抗氧化 活性氧基团 

分 类 号：R284[医药卫生—中药学]