基于单细胞RNA测序技术的皮肤学研究进展  

Research Progress of Single-cell RNA Sequencing Technology in Dermatological Applications

作　　者：蔺元斌 吕天琦 王宇辉 李琳 吴爱国 曹张磊 郑建萍 LIN Yuan-Bin;LYU Tian-Qi;WANG Yu-Hui;LI Lin;WU Ai-Guo;CAO Zhang-Lei;ZHENG Jian-Ping(Cixi Institute of Biomedical Engineering,Ningbo Institute of Materials Technology and Engineering,Chinese Academy of Sciences,Ningbo 315300,China;School of Material Science and Engineering,Shanghai University,Shanghai 200444,China)

机构地区：[1]中国科学院宁波材料技术与工程研究所慈溪生物医学工程研究所,宁波315300 [2]上海大学材料科学与工程学院,上海200444

出　　处：《分析化学》2022年第7期985-994,共10页Chinese Journal of Analytical Chemistry

基　　金：浙江省自然科学基金项目(No.LY20B050003);宁波市3315创新团队项目(No.2019A-14-C);宁波市科技重大专项项目(No.2016C50009)资助。

摘　　要：单细胞RNA测序技术是近年发展起来的一种高通量测序技术,该技术可在单个细胞层面上研究细胞基因表达和识别细胞异质性,极大地拓宽了研究者对皮肤发育、肿瘤产生和免疫应答等皮肤生物学机制的理解。近年来,皮肤方面的单细胞RNA测序研究系统地揭示了皮肤细胞的异质性,阐明了一些皮肤病的发病机制,并确定了多种临床皮肤病潜在的新型治疗靶点,为皮肤病的诊断和治疗提供了指导。本文详细综述了单细胞RNA测序技术和该技术在皮肤学领域中的最新研究进展,并探讨了该领域未来面临的主要挑战和发展的前景。Single-cell RNA sequencing technology is a high-throughput sequencing technology developed in recent years.It can study cellular gene expression and identify cell heterogeneity at the single cell level,broadening people′s understanding of skin development,tumor generation and immune response.Single-cell RNA sequencing studies in skin have systematically revealed the heterogeneity of skin cells,elucidated the pathogenesis of various skin diseases,and identified potential new therapeutic targets for a variety of clinical skin diseases,thus providing guidance for diagnosis and treatment.This article reviewed in detail the single-cell RNA sequencing technology and the latest research progress of this technology in the field of dermatology,aiming to improve the treatment level of skin diseases,and discussed the main challenges for the future development of this field.

关 键 词：单细胞RNA测序技术 皮肤学 文库制备 基因数据分析 评述 

分 类 号：R751[医药卫生—皮肤病学与性病学]