基于COF-JUZ2固定构象的左旋青霉胺分子印迹电化学传感器的制备和应用  

Construction and Application of L-Penicillamine Molecular Imprinted Electrochemical Sensor Based on Covalent Organic Framework-JUZ2 Fixed Conformation

作　　者：张连明 黎舒怀[1] 罗奎[2] 戴宇宁 李建平 ZHANG Lian-Ming;LI Shu-Huai;LUO Kui;DAI Yu-Ning;LI Jian-Ping(Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables,Analysis and Test Center of Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;Key Laboratory of New Technology of Food Safety Testing of Guangxi University,College of Chemistry and Bioengineering,Guilin University of Technology,Guilin 541004,China)

机构地区：[1]中国热带农业科学院分析测试中心,海南省热带果蔬质量安全重点实验室,海口571101 [2]桂林理工大学化学与生物工程学院,食品安全检测新技术广西高校重点实验室,桂林541004

出　　处：《分析化学》2022年第7期995-1004,共10页Chinese Journal of Analytical Chemistry

基　　金：海南省热带果蔬质量安全重点实验室开放课题项目(No.KFKT2020001);国家自然科学基金项目(No.21765006);广西自然科学基金项目(No.2018GXNSFAA138145);广西科技计划项目(No.桂科AD 19110059);桂林理工大学科研启动经费项目(No.GUTQDJJ2016024)资助。

摘　　要：采用共价有机框架化合物COF-JUZ2选择性固定手性分子空间构象,以改善印迹孔穴结构并增加识别位点,基于此构建了一种对青霉胺对映体具有高效分辨识别能力的分子印迹手性识别体系。以左旋青霉胺(L-Pen)为模板分子,与COF-JUZ2相互作用形成复合物后,以壳聚糖作为载体,采用电沉积方法固定在金电极表面,以邻苯二胺为功能单体电聚合制备分子印迹聚合物膜,洗脱L-Pen后,制备得到对L-Pen具备高分辨识别能力的手性分子印迹传感器。利用电化学方法对传感器制备条件及检测条件进行了优化。结果表明,当沉积时间为300 s,以甲醇-乙酸-超纯水(8∶1∶1,V/V)混合溶液为洗脱剂洗脱5 min,重吸附时间为30 min时,传感器性能最优。传感器响应信号强度与L-Pen浓度的对数在1.0×10^(-15)~1.0×10^(-9)mol/L范围内呈良好的线性关系,检出限(S/N=3)为7.91×10^(-16)mol/L。本研究通过引入COF-JUZ2,制备的传感器对D-Pen和L-Pen的分离效果提高了13.4倍。传感器成功用于药物中L-Pen的测定,回收率在92.0%~102.0%之间。The improvement of sensor selection recognition ability is crucial to the performance of molecular imprinted sensors.Herein,a molecularly imprinted polymer-based sensor was developed for L-penicillamine(L-Pen)detection based on covalent organic framework compounds COF-JUZ2.The molecular imprint chiral recognition sensor with efficient separation and recognition ability was constructed via selectively fixing the conformation of chiral molecules by COF-JUZ2 to increase the recognition sites.The sensor showed the optimal detection performance for L-Pen when using the mixed solution of ultrapure water,acetic acid and methanol(1∶1∶8,V/V)as eluant and the COF-JUZ2-CS deposition time,electropolymerization cycle,eluent time and rebind time were 300 s,15,5 and 30 min,respectively.The sensor showed an excellent linear relationship with L-Pen concentration in the range of 1.0×10^(-15)to 1.0×10^(-9)mol/L,with a detection limit of 7.91×10^(-16)mol/L.Due to introduction of COF-JUZ2,the separation and recognition performance of the sensor for D-Pen and L-Pen was increased by 13.4 folds.The sensor was applied to determination of L-Pen in real sample,with recoveries of 92.0%-102.0%.

关 键 词：分子印迹 手性分离 选择性 共价有机框架化合物 L-青霉胺 

分 类 号：O657.1[理学—分析化学] TQ460.72[理学—化学]