基于分子键裂的高频压电石英适配体生物传感器检测肌红蛋白  

Detection of Myoglobin by High Frequency Piezoelectric Quartz Aptamer Biosensor Based on Molecular Bond Rupture Technology

作　　者：余建芳 司士辉[1] 周卓 王桢昌 陈金华[2] YU Jian-Fang;SI Shi-Hui;ZHOU Zhuo;WANG Zhen-Chang;CHEN Jin-Hua(College of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China;State Key Laboratory of Chemical Biosensing and Metrology,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China)

机构地区：[1]中南大学化学化工学院,长沙410083 [2]湖南大学化学化工学院化学生物传感与计量学国家重点实验室,长沙410082

出　　处：《分析化学》2022年第7期1065-1071,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.2172810)资助。

摘　　要：基于调幅谐振压电石英体表面分子键裂传感技术和双适配体夹心法构建了一种检测肌红蛋白(Myo)的生物传感器。将巯基修饰的适配体Ⅰ通过Au—S键固定在石英晶体微天平(QCM)的金电极表面以特异性结合Myo,再结合适配体Ⅱ-磁珠进行质量放大。对于基频50 MHz镀金石英晶体,在谐振频率下增加激励电压峰值为8 V,结合在压电石英体表面的适配体-磁珠会被甩脱除去,发生分子键裂过程,晶体谐振频率上升,从而实现对Myo的特异性检测。在优化的实验条件下,检测Myo的线性范围为1.0~500 ng/mL,检出限(3σ)为0.38 ng/mL,实际血清样本中加标回收率为96.4%~104.0%。与常规QCM传感技术相比,本传感方法的信号获取过程简单、快速、易操作。基于分子键裂机制的QCM生物传感器具有良好的实用性和推广价值。A biosensor for detection of myoglobin based on the technology of molecular bond rupture of piezoelectric quartz crystal excited by amplitude modulation resonant and the method of double aptamer sandwich were developed.The aptamerⅠwas coated onto the gold electrode surface of quartz crystal microbalance(QCM)through Au—S bond,and specifically bound to myoglobin,which coupled with aptamerⅡ-magnetic beads to enhance the mass response.When the peak-to-peak value of the excitation voltage was increased to 8 V at the resonant frequency for 50 MHz gold-plated quartz crystal,the layer of aptamerⅡ-magnetic beads bonded to the surface would be removed,resulting in the occurrence of molecular bond rupture process and the increase of the resonant frequency,and the QCM aptamer biosensor with high specificity for detection of myoglobin was developed.Under the optimized conditions,the linear range of detection of myoglobin was 1.0-500 ng/mL,and the detection limit(3σ)was 0.38 ng/mL.The recoveries of myoglobin in human serum sample ranged from 96.4%to 104.0%.Compared to traditional QCM sensing technology,the sensing method established here was simple,fast,and easy to operate.Besides,the aptamer biosensor based on molecular bond rupture technology had practical and market application value.

关 键 词：石英晶体微天平 分子键裂 肌红蛋白 适配体 

分 类 号：R313[医药卫生—基础医学]