超声引导肝癌裸鼠异位种植瘤不完全消融模型的建立  

Establishment of ultrasound-guided incomplete ablation model of ectopic implanted tumor of liver cancer in nude mice

作　　者：吴林永 赵羽佳 庞锦树 廖蔚 陈玉姬 何云[1] 杨红[1] Wu Linyong;Zhao Yujia;Pang Jinshu;Liao Wei;Chen Yuji;He Yun;Yang Hong(Department of Medical Ultrasound,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院超声医学科,南宁530021

出　　处：《中华超声影像学杂志》2022年第5期440-445,共6页Chinese Journal of Ultrasonography

基　　金：国家自然科学基金(81860319,81960329)。

摘　　要：目的建立良好的肝癌裸鼠异位种植瘤不完全消融模型,探索残余癌分子景观的改变。方法将8只免疫缺陷BALB/c裸鼠以MHCC97-H肝癌细胞系建立异位种植瘤模型,简单随机分为实验组和对照组,每组4只。实验组进行模拟临床肝癌不完全消融,对照组仅进行假消融。通过超声诊断仪、热成像相机、大体病理、HE染色、高通量全转录组测序评估模型间的差异性改变。结果肝癌裸鼠异位种植瘤全部获得成功。实验组热成像相机监测针尖周围肿瘤温度位于50~73.9℃。与对照组相比,实验组HE染色大多显示为坏死区域-变性区域-肿瘤细胞区域共存的现象,坏死区域为(23.75±13.77)%,变性区域为50%(30%)。高通量全转录组测序显示体内外不完全消融模拟模型中存在数百个交集稳定分子景观。结论通过成功建立肝癌不完全消融残余癌的裸鼠异位种植模型,可为分子层面上研究不完全消融残余癌的生物学特性提供条件。Objective To establish a good model of incomplete ablation of ectopic implanted tumor of liver,and explore the changes in the molecular landscape of residual cancer,cancer in nude mice.Methods Eight immunodeficient BALB/c nude mice were used to establish an ectopic tumor model with the MHCC97-H hepatoma cell line,and they were randomly divided into experimental group and control group,with 4 mice in each group.The experimental group underwent simulated clinical incomplete ablation,and the control group only underwent false ablation.The differences between the models were evaluated by ultrasonic diagnostic equipment,thermal imaging cameras,HE staining and high-throughput whole transcriptome sequencing.Results Liver cancer ectopic implantations in nude mices were all successful.The experimental group showed that the temperature of the tumor around the tip of the needle monitored by the thermal imaging camera was at 50-73.9℃.Compared with the control group,the HE staining of the experimental group mostly showed the coexistence of necrotic area-degeneration area-tumor cell area.The necrosis area was(23.75±13.77)%,and the degeneration area was 50%(30%).High-throughput whole transcriptome sequencing revealed that there were hundreds of overlapping stable molecular landscapes in the incomplete ablation simulation model both in vivo and in vitro.Conclusions By establishing an ectopic implantion model of nude mice with incomplete ablation of residual liver cancer,it can provide a basis for studying the biological characteristics of incomplete ablation of residual cancer at the molecular level.

关 键 词：不完全消融 肝癌 异位种植 动物模型 分子景观 

分 类 号：R445.1[医药卫生—影像医学与核医学] R735.7[医药卫生—诊断学]