盾叶薯蓣须根响应低磷胁迫的GC-MS代谢产物及基因表达特征分析  

GC-MS Metabolites and Gene Expression Characteristics in Fibrous Roots of Dioscorea zingiberensis in Response to Low Phosphorus Stress

作　　者：谢彩侠 崔锦鹏 李雅静 张娟 刘庆普 龚海燕 雷敬卫 王丰青[2] XIE Caixia;CUI Jinpeng;LI Yajing;ZHANG Juan;LIU Qingpu;GONG Haiyan;LEI Jingwei;WANG Fengqing(Henan Provincial Engineering Technology Research Center for Quality Control and Evaluation of Traditional Chinese Medicine,Scholl of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Henan Agricultural University,Zhengzhou 450032,China)

机构地区：[1]河南中医药大学药学院,河南省中药质量控制与评价工程技术研究中心,郑州450046 [2]河南农业大学,郑州450032

出　　处：《中国实验方剂学杂志》2022年第13期189-197,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省高等学校重点科研项目(20A360016)。

摘　　要：目的:探讨盾叶薯蓣须根在低磷胁迫下的代谢产物及基因表达特征。方法:设置重度胁迫组、中度胁迫组与正常组模拟盾叶薯蓣低磷胁迫实验,在胁迫初期采取盾叶薯蓣须根,分别利用气相色谱-质谱联用技术(GC-MS)衍生化及RNA-seq技术对其代谢产物和转录组特征进行分析,通过对不同处理代谢产物的多元统计分析、差异表达基因的功能分析及数据挖掘,筛选低磷胁迫下盾叶薯蓣须根产生的代谢标志物,分析差异表达基因的代谢通路特征。结果:从盾叶薯蓣须根中共检测到116个GC-MS代谢产物,不同低磷处理下盾叶薯蓣须根的代谢特征存在明显差异,利用正交偏最小二乘判别分析(OPLS-DA)模型从不同处理须根的代谢产物中筛选到6个差异代谢物,主要为糖类、醇类等成分,这些物质可能是盾叶薯蓣须根响应低磷胁迫的代谢标志物;重度胁迫组与正常组中筛选到的差异基因主要富集到过氧化物酶途径、磷酸盐和次磷酸盐代谢途径,重度胁迫组与中度胁迫组中筛选到的差异基因主要富集到谷胱甘肽代谢途径及磷酸戊糖途径;从须根中筛选出177个潜在响应低磷胁迫的差异基因,涉及萜类骨架、肌醇生物合成等多个途径,这与盾叶薯蓣须根部位响应低磷胁迫的代谢差异物多为糖类与肌醇等物质相吻合。结论:低磷胁迫下盾叶薯蓣须根部位的代谢产物及基因表达均发生了相应的应答,差异代谢物与差异表达基因具有密切的关系,该研究为盾叶薯蓣响应低磷胁迫的分子机制提供理论依据。Objective: To investigate the metabolites and gene expression characteristics in fibrous roots of Dioscorea zingiberensis in response to low phosphorus stress. Method: The severe stress group,the moderate stress group,and the normal group were set up to stimulate the low phosphorus stress experiment. The fibrous roots of D. zingiberensis were collected during initial stress. The metabolites and transcriptomic characteristics were analyzed by gas chromatography-mass spectrometry(GC-MS)derivatization and RNA-seq techniques. Through multivariate statistical analysis of metabolites treated by different methods,functional analysis of differentially expressed genes,and data mining,the metabolism markers produced in fibrous roots of D. zingiberensis under low phosphorus stress were screened out,and the metabolic pathway characteristics of different genes were analyzed. Result: A total of 116 GC-MS metabolites were detected from the fibrous roots of D. zingiberensis. The metabolic characteristics of fibrous roots of D. zingiberensis under different low phosphorus treatments were obviously different. Orthogonal partial least squares discriminant analysis(OPLSDA)model was used to screen six differential metabolites represented by sugars and alcohols from metabolites of fibrous roots treated with different methods,and these components were presumedly metabolism markers of fibrous roots of D. zingiberensis in response to low phosphorus stress. The differential genes screened out from the severe stress group and the normal group were mainly enriched in peroxidase pathway,phosphate and hypophosphate metabolism pathway,while the differential genes screened out from the severe stress group and the moderate stress group were mainly enriched in glutathione metabolism pathway and phosphopentose pathway. A total of 177 differential genes in response to low phosphorus stress were screened out from fibrous roots,involving many pathways such as terpenoid skeleton and inositol biosynthesis,which was consistent with the fact that the met

关 键 词：盾叶薯蓣 低磷胁迫 气相色谱-质谱联用技术(GC-MS) RNA-seq 差异代谢物 差异基因 

分 类 号：R284.2[医药卫生—中药学] R289[医药卫生—中医学] R22R2-031R33