黄麻CAMTA1基因的克隆、表达及其与AP2/ERF类转录因子相互作用的分析  

Cloning,Expression and Interaction with AP2/ERF Transcription Factor Analysis of CAMTA1 Gene from Corchorus capsularis

作　　者：张高阳[1] 邓接楼[1] 徐建堂 张超 周越 叶水烽 伍应保 杨锌 蒋蕾 黄思齐[4] 李德芳[4] ZHANG Gao-Yang;DENG Jie-Lou;XU Jian-Tang;ZHANG Chao;ZHOU Yue;YE Shui-Feng;WU Ying-Bao;YANG Xin;JIANG Lei;HUANG Si-Qi;LI De-Fang(College of Life Sciences,Shangrao Normal University,Shangrao 334001,China;College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Jiangxi Medical College,Shangrao 334001,China;Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha 410000,China)

机构地区：[1]上饶师范学院生命科学学院,上饶334001 [2]福建农林大学农学院,福州350028 [3]江西医学高等专科学校,上饶334001 [4]中国农业科学院麻类研究所,长沙410000

出　　处：《农业生物技术学报》2022年第6期1087-1095,共9页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31860396,32160457);江西省自然科学基金(20212BAB205026);上饶市科技支撑项目(2020L008)。

摘　　要：钙调蛋白转录因子(calmodulin-binding transcription factor,CAMTA)在植物生长发育、响应生物和非生物逆境胁迫过程中发挥重要调控作用。黄麻(Corchorus capsularis)是一种十分重要的草本韧皮纤维经济作物,为研究黄麻中CAMTA在干旱逆境响应中的作用,本研究以圆果黄麻’黄麻179’为材料,根据黄麻基因组测序的CAMTA基因序列(Cc.02G0003260)设计引物,用RT-PCR技术克隆获得CAMTA基因,命名为CAMTA1(GenBank No.OK415793)。序列分析表明,CAMTA1基因包含13个外显子,12个内含子,其开放阅读框为3051 bp,编码1个含1016个氨基酸的蛋白质,具有CAMTA类蛋白所具有的CG-1结构域、TIG结构域、ANK重复序列以及IQ基序。通过蛋白序列比对发现CAMTA1与锦葵科植物的CAMTA蛋白质同源性较高。qPCR分析表明CAMTA1基因在根、茎、叶和果皮中均有表达,在干旱胁迫诱导后6.0 h时叶片中的CAMTA1基因表达量达到最高。荧光素酶蛋白互补实验表明,CAMTA1能与另一类逆境响应转录因子AP2/ERF相互作用,共同调控植物的生长发育及响应生物与非生物胁迫。研究结果为进一步阐明CAMTA1基因参与植物干旱响应的分子机理提供参考。Calmodulin-binding transcription factor(CAMTA)plays an important role in the regulation of plant growth and development and response to abiotic and biological stress.Jute(Corchorus capsularis)is an important commercial crop of bast fiber.In order to study the role of CAMTA in response to drought stress in jute,the’Corchorus capsularis 179’was used as the material,primers were designed based on the sequence of CAMTA gene(CC.02G0003260)from the jute genome.The CAMTA gene was obtained by RT-PCR and named as CAMTA1(OK415793).Sequence analysis showed that the CAMTA1 gene contained 13 exons and 12introns,Its open reading frame was 3051bp and encoded a protein containing 1016 amino acids,which had the CG-1 domain,TIG domain,ANK repeat sequence and IQ motif of CAMTA proteins.Protein sequence alignment indicated that CAMTA1 had high homology with the protein of Malvaceae plants.q PCR analysis showed that CAMTA1 gene was expressed in root,stem,leaf and pericarp.The expression of CAMTA1 gene in leaves reached the highest at 6.0 h after drought stress induction.Fluorescence enzyme protein complementation assay suggested that CaMTA1 interacted with another stress-responsive transcription factor AP2/ERF to regulate plant growth,development and respond to both abiotic and biological stresses.These results provide a theoretical basis for further elucidating the molecular mechanism of CAMTA1 gene involved in plant drought response.

关 键 词：黄麻 钙调蛋白转录因子(CAMTA) 干旱胁迫 蛋白互作 

分 类 号：S563.5[农业科学—作物学]