桃纤维素合成酶(CesA/Csl)基因超家族成员鉴定及表达分析  

Identification and Expression Analysis of Cellulose Synthase(CesA/Csl)Gene Superfamily Members in Peach(Prunus persica)

作　　者：张兴珍 康同洋 暴茹 刘鑫丽 覃荣玲 赵彩平[1] ZHANG Xing-Zhen;KANG Tong-Yang;BAO Ru;LIU Xin-Li;QIN Rong-Ling;ZHAO Cai-Ping(College of Horticulture,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学园艺学院,杨凌712100

出　　处：《农业生物技术学报》2022年第6期1096-1111,共16页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31572079);大学生创新创业训练项目(S202010712716)。

摘　　要：纤维素合成酶基因超级家族包括纤维素合成酶(cellulose synthase,CesA)和类纤维素合成酶(cellulose synthase-like,Csl)基因家族,参与纤维素、半纤维素和果胶等多糖的生物合成,在植物细胞壁的形成中起着重要作用。本研究在桃(Prunus persica)基因组中鉴定出36个PpCesA/Csl基因家族成员,分别编码394~1527个氨基酸,蛋白质平均分子量为44.85~171.80 kD,内含子数量为4~16,包含20个不同的基序。PpCesA/Csl蛋白的亚细胞定位具有多样性,可在叶绿体、高尔基体、细胞膜、细胞质及线粒体中定位。系统进化分析表明,PpCesA/Csl分为7个亚族(CesA,CslA,CslB,CslC,CslD,CslE和CslG)。所有PpCesA/Csl蛋白都含有7个跨膜结构域,其中27个成员含有纤维素合成酶结构域PF03552,9个成员含有糖基转移酶家族2结构域PF00535;另外,除PpCesA2外其他8个PpCesA亚族成员均含有锌指结构域zfUDP,除PpCslD1外其他4个CslD亚族成员均含有锌指结构域zf-RING_4。PpCesA/Csl蛋白的主要保守活性位点是天冬氨酸D、D、D残基,其次是QxxRW基序。通过qPCR分析PpCesA6、PpCesA8、PpCslD2、PpCslE7、PpCslG1在不同组织、果实不同发育期和贮藏期的表达特性。结果显示,PpCesA6和PpCslG1在生殖器官花和果实中表达量较高,PpCesA8在果实和新梢中表达量较高,而PpCslD2在果实和根以及PpCslE7在根和花中表达量较高。在果实发育期,PpCesA6、PpCesA8和PpCslD2均在盛花后65 d达到表达峰值;而PpCslE7和PpCslG1分别在盛花后80 d和盛花后35 d出现表达量高峰。在果实贮藏期,PpCslD2和PpCslE7在两品种中均呈现上调表达趋势,而PpCesA8呈下调表达趋势;PpCslG1在’秦王’果实的贮藏前期高表达,而在’沙红’果实贮藏期表达量极低。本研究为桃和蔷薇科其他植物CesA/Csl基因的进一步功能研究提供了基础资料。The cellulose synthase(CesA)and cellulose synthase-like(Csl)gene families belonging to the cellulose synthase gene superfamily are responsible for the biosynthesis of cellulose,hemicellulose and pectin polysaccharides of the plant cell wall,and play critical roles in plant development,growth and evolution.In this study,36 PpCesA/Csl gene family members were identified in the Prunus persica genome,encoding 394 to 1527 amino acids,respectively.The average molecular weight of the proteins ranged from 44.85 to 171.80kD,and the number of introns ranged from 4 to 16,containing 20 different motifs.The subcellular localization of PpCesA/Csl protein was diverse and can be located in chloroplasts,golgi bodies,cell membranes,cytoplasm and mitochondria.Phylogenetic analysis showed that PpCesA/Csl could be divided into 7 subfamilies(CesA,CslA,CslB,CslC,CslD,CslE and CslG).All PpCesA/Csl proteins contained 7 transmembrane domains,of which 27 members contained cellulose synthase domain PF03552 and 9 members contained glycosyltransferase family 2 domain PF00535.In addition,the other 8 PpCesA subgroup members except PpCesA2 contained zinc finger domain zf-UDP,and the other 4 CslD subgroup members except PpCslD1 contained zinc finger domain zf-RING_4.The main conserved active sites of PpCesA/Csl protein were aspartic acid D,D,D residues,followed by QxxRW motif.Most PpCesA/Csl family members contained complete amino acid sequences of this active site.The results indicated that most of the CesA/Csl genes from peach were closely related to genes in Arabidopsis thaliana,but these families had unique characteristics in terms of their gene structure,chromosomal localization,phylogeny,and deduced protein sequences,suggesting that they had evolved through different processes.The transcriptome data of’Qian Jianbai’peach during storage were used to analyze the expression characteristics of peach CesA/Csl members in mature fruit.The results showed that most peach CesA/Csl members were not expressed or very low expression level during the

关 键 词：桃 纤维素合成酶超家族 果实 表达模式 

分 类 号：S662.1[农业科学—果树学]