黔北麻羊CYP17A1、CYP19A1基因组织表达、功能预测及与产羔数的关联性分析  

Tissue Expression,Functional Prediction of CYP17A1 and CYP19A1 Genes in Qianbei Ma Goat(Capra hircus)and Its Association Analysis with Litter Size

作　　者：张艳[1,2,3] 陈祥 周志楠[1,2,3] 付开斌[1,2,3] 王忠 李世军 杨沛方[1,2,3] 惠茂茂 ZHANG Yan;CHEN Xiang;ZHOU Zhi-Nan;FU Kai-Bin;WANG Zhong;LI Shi-Jun;YANG Pei-Fang;HUI Mao-Mao(Key Laboratory of Genetic Breeding and Breeding of Highland Mountain Animals,Ministry of Education,Guizhou University,Guiyang 550025,China;Key Laboratory of Genetic Breeding and Breeding of Animals,Guizhou Province,Guiyang 550025,China;School of Animal Science,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学高原山地动物遗传育种与繁殖教育部重点实验室,贵阳550025 [2]贵州省动物遗传育种与繁殖重点实验室,贵阳550025 [3]贵州大学动物科学学院,贵阳550025

出　　处：《农业生物技术学报》2022年第6期1140-1152,共13页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31760652);贵州省科技支撑计划项目(黔科合支撑[2021]一般151);贵州省肉羊产业技术体系项目(GZRYTX2019-01)。

摘　　要：细胞色素P450c17(cytochrome P450c17,CYP17A1)及细胞色素P450芳香化酶(cytochrome P450arom,CYP19A1)对卵泡发育、排卵、主要生殖激素分泌及合成均有重要作用,并影响动物的繁殖性能。本研究以健康黔北麻羊(Capra hircus)为研究对象,利用qPCR检测CYP17A1、CYP19A1基因的组织表达,以PCR直接测序法筛选SNP突变位点,通过建立一般线性模型分析SNPs对黔北麻羊不同胎次产羔数的影响;分析了CYP17A1、CYP19A1基因在各组织中的表达规律及SNPs与产羔数之间的关联性。结果显示,CYP17A1基因在单、多羔个体卵巢均表达最高,且极显著高于垂体、下丘脑、子宫、输卵管(P<0.01);CYP19A1基因在多羔卵巢及子宫表达较高,且均极显著高于对应单羔组织(P<0.01)。CYP17A1基因编码区共检测出7个SNPs,g.59G>C、g.128G>A、g.129A>G、g.164T>C均位于第1外显子,g.2045G>A位于第2外显子,g.4387G>A和g.4456C>T位于第6外显子;关联性分析表明,CYP17A1基因SNPs与黔北麻羊产羔数均不相关。CYP19A1基因编码区第9外显子检测出一个变异位点g.48818C>A,其AA基因型的产羔数显著高于CA基因型(P<0.05),与CC基因型产羔数差异不显著。结果表明,黔北麻羊CYP17A1、CYP19A1基因在多羔个体卵巢组织中均表达最高(P<0.01);CYP17A1基因SNPs与黔北麻羊产羔数之间不相关,CYP19A1基因g.48818C>A位点AA基因型150只羊中仅存在4只,因样本量少,还需验证。本研究为进一步探究CYP17A1、CYP19A1基因变异与动物繁殖繁育之间的关系提供了基础数据。CytochromeP450c17(CYP17A1)and cytochromeP450 aromatase(CYP19A1)were important for follicle development,ovulation and the secretion and synthesis of major reproductive hormones,and affected the reproductive performance of animals.In this study,healthy Qianbei Ma goat(Capra hircus)were taken as the research object.The tissue expression of CYP17A1 and CYP19A1 genes was detected by qPCR,and the SNP mutation sites were screened by PCR direct sequencing.The effect of SNPs on the lambing number of different parity of Qianbei Ma goat was analyzed by establishing a general linear model.The expression of CYP17A1 and CYP19A1 genes in various tissues and the correlation between SNPs and litter size were analyzed.The results showed that the expression of CYP17A1 gene was the highest in the ovary of single and multiple lambs,and was extremely significantly higher than that of pituitary,hypothalamus,uterus and fallopian tube(P<0.01).CYP19A1 gene expression was higher in the multi-lamb ovary and uterus,and both were higher than the corresponding single-lamb tissue(P<0.01).A total of 7 SNPs were detected in the CYP17A1 gene coded region.g.59G>C,g.128G>A,g.129A>G and g.164T>C were all located in the first exon,g.2045G>A was in the second exon and g.4387G>A and g.4456C>T were in the sixth exon.Correlation analysis showed that the SNPs of CYP17A1 gene were not correlated with the litter size of Qianbei Ma goat.A mutation site g.48818C>A was detected in the 9th exon of the coding region of CYP19A1 gene and the number of lambs in the AA genotype was significantly higher than that of the CA genotype(P<0.05),but not significantly different from that of the CC genotype.The results showed that the expression of CYP17A1 and CYP19A1 genes in Qianbei Ma goat was the highest in the ovarian tissue of multiple lambs(P<0.01).There were only 4 goats in 150 with AA genotype g.48818C>A site,which was not representative and needs to be verified.This study provides basic data for further exploring the relationship between CYP17A1 and CYP19A1gene vari

关 键 词：黔北麻羊 细胞色素P450c17(CYP17A1) 细胞色素P450芳香化酶(CYP19A1) 产羔数 SNP 

分 类 号：S827[农业科学—畜牧学]