口服脾多肽改善氨水诱导的小鼠咳嗽和体内炎症  

Oral lienal peptides improve ammonia-induced coughing and inflammation in mice

作　　者：毛姝英 金蔚 付思思 刘珂安琪 周志豪 王广基[1] 梁艳[1] MAO Shuying;JIN Wei;FU Sisi;LIU Keanqi;ZHOU Zhihao;WANG Guangji;LIANG Yan(Key Lab of Drug Metabolism and Pharmacokinetics,State Key Laboratory of Natural Medicines,China Pharmaceutical University,Nanjing 210009,Jiangsu,China)

机构地区：[1]中国药科大学药物代谢动力学重点实验室天然药物与活性组分国家重点实验室,江苏南京210009

出　　处：《中国临床药理学与治疗学》2022年第6期601-607,共7页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：河北省自然科学基金项目(H2021302001,H2020208022,H2020208025,C2021418001,H2021208006)。

摘　　要：目的:研究口服脾多肽对小鼠咳嗽和体内炎症的改善作用,旨在拓展免疫调节剂脾多肽在临床上的应用,为临床提供缓解咳嗽和炎症治疗的新策略。方法:采用浓氨水诱导小鼠咳嗽模型,每天记录小鼠6 min内的咳嗽频率和耐受潜伏期;采用HE染色、脾指数评估小鼠脾、肺的组织病理变化;采用ELISA检测小鼠脾、肺组织中的TNF-α、IL-1β、IL-6水平。结果:口服脾多肽可以一定程度上延长小鼠对浓氨水的耐受潜伏期,显著降低小鼠的咳嗽频率;HE染色结果表明口服脾多肽可以显著减少小鼠的肺泡表面积,改善肺部扩张;ELISA结果表明口服脾多肽降低脾、肺内的部分促炎因子水平。结论:脾多肽可以缓解氨水诱导的小鼠咳嗽和类似肺气肿症状,提高小鼠的机体免疫能力,改善小鼠的体内炎症。AIM:To study the effect of oral lienal polypeptide on cough and inflammation in mice,in order to expand the clinical application of immune modulator lienal polypeptide and provide a new strategy for relieving cough and inflammation.METHODS:The cough model of mice was induced by concentrated ammonia.The cough frequency and tolerance latency of mice within 6 minutes were recorded every day.The histopathological changes of spleen and lung were evaluated by HE staining and spleen index.TNF-α,IL-1β and IL-6 levels in spleen and lung of mice was detected by ELISA.RESULTS:Oral administration of spleen polypeptide could prolong the tolerance latency of mice to concentrated ammonia to a certain extent and significantly reduce the cough frequency of mice.HE staining showed that oral spleen polypeptide could significantly reduce the alveolar surface area and improve lung expansion in mice.The results of ELISA showed that oral spleen polypeptide decreased the levels of some proinflammatory factors in spleen and lung.CONCLUSION:Lienal polypeptide can alleviate cough and emphysema like symptoms induced by ammonia,improve immune ability and inflammation in mice.

关 键 词：脾多肽 氨水引咳 肺气肿 炎症 

分 类 号：R965.2[医药卫生—药理学]