新型鸭呼肠孤病毒SY株的分离鉴定及培养特性  

Isolation,Identification and Cultural Characteristics of a Novel Duck Reovirus Strain SY

作　　者：晁锦 卢凤英[1] 潘群兴[1] 孙华伟[1] 张敬峰[1] 董永毅 徐正军 祁克宗[2] 张小飞[1] CHAO Jin;LU Fengying;PAN Qunxing;SUN Huawei;ZHANG Jingfeng;DONG Yongyi;XU Zhengjun;QI Kezong;ZHANG Xiaofei(Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China;Jiangsu Provincial Center for Animal Disease Control and Prevention,Nanjing 210009,China)

机构地区：[1]江苏省农业科学院兽医研究所,南京210014 [2]安徽农业大学动物科技学院,合肥230036 [3]江苏省动物疫病预防控制中心,南京210009

出　　处：《中国动物传染病学报》2022年第3期48-54,共7页Chinese Journal of Animal Infectious Diseases

基　　金：国家重点研发计划项目(2017YFD0500804);江苏省农业科技自主创新资金项目(CX(17)3024,CX(19)3002);江苏现代农业(水禽)产业技术体系疾病防控创新团队(JATS[2018]222)。

摘　　要：本研究以肝脏出血和脾脏坏死为特征的病死鸭肝脏和脾脏组织接种SPF鸡胚进行病毒分离,并利用PCR方法进行病原鉴定,对分离病毒进行了回归雏鸭试验和S1基因测序分析。结果显示:该分离病毒为新型鸭呼肠孤病毒,并命名为NDRV SY株;将该分离病毒接种SPF鸡胚、BHK-21和Vero细胞进行传代培养,结果表明其能稳定适应鸡胚增殖和致鸡胚死亡,在BHK-21和Vero细胞两种传代细胞上也能良好增殖,并产生明显细胞病变。本研究为NDRV的疫苗和致病机制研究奠定了基础。In this study,affected tissues of the diseased ducks characterized by liver hemorrhage and spleen necrosis were inoculated into SPF chicken embryos for virus isolation and the isolated virus was analyzed by PCR,animal regression test and sequencing of S1 gene.The results showed that the isolated virus was a Novel duck reovirus designated as NDRV SY strain.The NDRV SY strain was passaged continuously in SPF chicken embryos,BHK-21 and Vero cells.The virus gradually adapted to grow in chicken embryos and caused death.It also propagated well on cell cultures and produced obvious cytopathy.Availability of the NDRV SY strain provided materials for the research of pathogenesis and vaccine development.

关 键 词：新型鸭呼肠孤病毒 分离 鉴定 培养特性 

分 类 号：S852.65[农业科学—基础兽医学]