血清Ⅰ型鸭甲型肝炎病毒VP3蛋白单克隆抗体的制备与初步应用  

Preparation and Preliminary Application of Monoclonal Antibody against VP3 Protein of Duck Hepatitis Virus Type 1

作　　者：陈长春[1] 王永娟[1] 崔平福 朱善元[1] CHEN Changchun;WANG Yongjuan;CUI Pingfu;ZHU Shanyuan(Jiangsu Agri-animal Husbandry Vocational College,Jiangsu Provincial Key Laboratory of Veterinary Bio-pharmaceutical High-tech Research,Taizhou 225300,China;Taizhou Customs,Taizhou 225300,China)

机构地区：[1]江苏农牧科技职业学院,江苏省兽用生物制药高技术研究重点实验室,泰州225300 [2]泰州海关,泰州225300

出　　处：《中国动物传染病学报》2022年第3期152-157,共6页Chinese Journal of Animal Infectious Diseases

基　　金：2019江苏省教育厅重大项目及校级配套支持项目(19KJA520008,NSFZP202103);2019“青蓝工程”中青年学术带头人资助项目(00000219011)。

摘　　要：为研制血清Ⅰ型鸭肝炎病毒主要的结构蛋白之一VP3蛋白的单克隆抗体,将鸭肝炎病毒免疫BALB/c小鼠,利用原核表达的Ⅰ型鸭肝炎病毒VP3重组蛋白作为抗原进行筛选以及3次亚克隆后,获得了一株(2G3)能够稳定分泌抗VP3蛋白单克隆抗体的杂交瘤细胞,单克隆抗体亚类鉴定为IgG1,ELISA检测腹水效价为1∶12800。鉴定结果表明制备的单克隆抗体效价高,反应原性强,为后续建研制血清Ⅰ型鸭甲型肝炎病毒的快检试剂盒及建立病原及抗体检测方法奠定基础。In order to prepare mAbs against DHAV-1 VP3 protein,BALB/c mice were immunized with the purified DHAV-1 antigen and the prokaryotically expressed DHAV-1 VP3 recombinant protein was used as antigen for screening and subcloning.A hybridoma cell line(2G3)that stably secreting mAbs specific for DHAV-1 VP3 protein was obtained after three cycles of subcloning.The subclass of this mAb was identified as IgG1 and its ascitic titer was 1∶12800 as tested by ELISA.The availability of the specific mAb laid a foundation for the further development of rapid detection for Duck hepatitis A virus type 1.

关 键 词：Ⅰ型鸭甲型肝炎病毒 VP3基因 单克隆抗体 

分 类 号：S859.797[农业科学—临床兽医学]