外源茉莉酸甲酯和赤霉素对茜草生长、相关酶活性及主要活性成分含量的影响研究  

Effects of exogenous methyl jasmonate and gibberellins on growth,related enzyme activities and contents of active constituents of Rubia cordifolia

作　　者：彭亮 罗瑶 李翡 沈霞 周可心 颜永刚 张健健 胡本祥 杨冰月 PENG Liang;LUO Yao;LI Fei;SHEN Xia;ZHOU Ke-xin;YAN Yong-gang;ZHANG Jian-jian;HU Ben-xiang;YANG Bing-yue(Shaanxi Qinling Application Development and Engineerig Center of Chinese Herbal Medicine,College of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,China;Key Laboratory for Research of"Qin medicine"of Shaanxi Administration of Traditional Chinese Medicine,Xi’an 712046,China;Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712000,China;Shaanxi Institute of International Trade and Commerce,Xi’an 712046,China)

机构地区：[1]陕西中医药大学药学院/陕西省秦岭中草药应用开发工程技术研究中心,陕西西安712046 [2]“秦药”研发重点实验室,陕西西安712046 [3]陕西中医药大学附属医院,陕西咸阳712000 [4]陕西国际商贸学院,陕西西安712046

出　　处：《中草药》2022年第11期3463-3471,共9页Chinese Traditional and Herbal Drugs

基　　金：陕西省教育厅项目(19JK0225);陕西省中医药管理局项目(2021-QYZL-02);2018年医疗服务与保障能力提升补助资金(中医药事业传承与发展部分)“全国中药资源普查项目”(财社[2018]43号);陕西中医药大学“秦药”品质评价及资源开发学科创新团队项目(2019-QN01)。

摘　　要：目的以茜草Rubia cordifolia为研究对象,探讨施加外源不同浓度茉莉酸甲酯(methyl jasmonate,MeJA)和赤霉素(gibberellins,GA3)对茜草幼苗生长、相关酶活性及主要活性成分含量的影响。方法取大小一致的1月龄茜草实生苗转入霍格兰营养液中培养30 d后,分别添加不同浓度的MeJA(25、50、100、150μmol/L)和GA3(50、100、200、400 mg/L)继续培养7 d,测定茜草的地上、地下部分长度和干鲜质量、渗透调节物质含量、抗氧化酶活性及5种主要活性成分的含量。结果在所选择浓度范围内,MeJA对茜草幼苗生长的影响为低浓度促生,高浓度抑制;GA3的影响则表现为低浓度抑制,高浓度促生。生理活性方面,50μmol/L MeJA和400 mg/L GA3处理下,叶绿素、游离脯氨酸和总蛋白含量显著增高;过氧化物酶(peroxidase,POD)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、丙二醛(malondialdehyde,MDA)活性因激素种类及浓度而呈差异性,150μmol/L MeJA和50 mg/L GA3处理下活性较强。MeJA和GA3的浓度对茜草活性成分积累影响很大,50~150μmol/L MeJA可以显著促进茜草幼苗中茜草素、羟基茜草素、1,8-二羟基蒽醌、甲基异茜草素和大叶茜草素的积累,且随着MeJA浓度的增加促进作用越强(P<0.05);200、400 mg/L GA3处理可以明显增强活性成分的积累(P<0.05)。结论茜草幼苗的生长发育、生理活性及主要活性成分的积累受外源MeJA和GA3浓度的影响,50μmol/L MeJA和400 mg/L GA3诱导能够显著提高茜草幼苗生物量及促进茜草幼苗中茜草素、羟基茜草素、1,8-二羟基蒽醌、甲基异茜草素和大叶茜草素的积累。Objective To study the effects of exogenous methyl jasmonate(MeJA)and gibberellins(GA3)on seedling growth,related enzyme activities and the contents of main active components of Rubia cordifolia.Methods One-month-old seedlings of R.cordifolia were cultured in Hoagland nutrient solution for 30 d,and then added with different concentrations of MeJA(25,50,100,150μmol/L)and GA3(50,100,200,400 mg/L)for 7 d,respectively.The length,dry and fresh weight,osmotic adjustment substance content,antioxidant enzyme activities and five main active components of R.cordifolia were measured.Results The effect of MeJA on the growth of R.cordifolia seedlings was promoted by low concentration and inhibited by high concentration in the concentration range selected in this experiment.The effect of GA3 was inhibited by low concentration and promoted by high concentration.In physiological activities,the contents of chlorophyll,free proline and total protein increased significantly under 50μmol/L M eJA a nd 400 mg/L GA3 t reatment;The a ctivities o f P OD,S OD,C AT a nd the c ontent o f M DA were d ifferent with t he hormone types and concentrations.The activities were stronger under 150μmol/L MeJA and 50 mg/L GA3 treatment.The concentration of MeJA and GA3 had a great influence on the accumulation of active components in R.cordifolia.The accumulation of alizarin,purpurin,1,8-dihydroxyanthraquinone,rubiadin and mollugin was significantly promoted by 50—150μmol/L MeJA in R.cordifolia.seedlings,and the promotion was stronger with the increase of MeJA concentration(P<0.05);the accumulation of active components was significantly enhanced by 200 and 400 mg/L GA3 treatment(P<0.05).Conclusion The growth,physiological activities and accumulation of main active components of R.cordifolia.seedlings are affected by the concentration of exogenous MeJA and GA3.MeJA(50μmol/L)and GA3(400 mg/L)can significantly increase the biomass of R.cordifolia seedlings and promote the accumulation of alizarin,purpurin,1,8-dihydroxyanthraquinone,rubiadin and mo

关 键 词：茜草 茉莉酸甲酯 赤霉素 抗氧化酶 渗透调节物质 茜草素 羟基茜草素 1 8-二羟基蒽醌 甲基异茜草素 大叶茜草素 

分 类 号：R286.2[医药卫生—中药学]