分心木化学成分及其体外抗肿瘤活性研究  

Chemical constituents from Diaphragma Juglandis Fructus and its antitumor activities

作　　者：陈良[1,3,4] 葛朝伦 魏鸿雁[2] 马晓玲 买迪娜 石磊岭[2] CHEN Liang;GE Chao-lun;WEI Hong-yan;MA Xiao-ling;MAI Di-na;SHI Lei-ling(State Key Laboratory of Pathogenesis,Prevention and Treatment of High Incidence Diseases in Central Asia,Department of Pharmacy,Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University,Urumqi 830000,China;Xinjiang Institute of Chinese and Ethnic Medicine,Urumqi 830002,China;Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi 830011,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]新疆医科大学附属中医医院,药学部,省部共建中亚高发病成因与防治国家重点实验室,新疆乌鲁木齐830000 [2]新疆维吾尔自治区中药民族药研究所,新疆乌鲁木齐830002 [3]中国科学院新疆理化技术研究所,新疆乌鲁木齐830011 [4]中国科学院大学,北京100049

出　　处：《中草药》2022年第12期3595-3600,共6页Chinese Traditional and Herbal Drugs

基　　金：省部共建中亚高发病成因与防治国家重点实验室开放课题资助项目(SKL-HIDCA-2020-ZY12);上海合作组织科技伙伴计划及国际科技合作计划项目(2020E01011)。

摘　　要：目的 研究分心木DiaphragmaJuglandisFructus的化学成分及其体外抗肿瘤活性。方法 综合应用多种色谱技术方法进行分离纯化,结合现代波谱技术及理化性质进行结构鉴定;采用MTT法检测化合物对人子宫颈癌He La细胞和人胃癌HGC-27细胞的体外抑制活性。结果 从分心木95%甲醇提取物中分离得到16个化合物,分别鉴定为isosclerone(1)、engelhardione(2)、5-dehydroxy-octahydro-demethoxycurcumin-A(3)、(E)-3,3′-dimethoxy-4,4′-dihydroxystilbene(4)、blumenol A(5)、(+)-erythro-7-O-ethylguaiacylglycerol(6)、threo-3-(4-hydroxy-3,5-dimethoxyphenyl)-3-ethoxypro-pane-1,2-diol(7)、(6R,9R)-blumenolB(8)、hexahydro-demethoxycurcumin-A(9)、blumenolC(10)、3,4-二羟基苯甲酸乙酯(11)、(+)-异落叶松脂醇(12)、胡桃宁B(13)、heptanoid(14)、4,17-dimethoxy-2-oxatricyclo [13.2.2.13,7]eicosa-3,5,7(20),15,17,18-hexaene-10,16-diol(15)、alternariol 9-methyl ether(16)。结论 化合物1~16均为首次从分心木中分离得到,化合物9和13对He La细胞具有抑制作用,其半数抑制浓度(medianinhibitoryconcentration,IC50)分别为25.33、16.29μmol/L,而化合物13对HGC-27细胞表现出一定的活性。Objective To study the chemical constituents and their antitumor activities from Diaphragma Juglandis Fructus.Methods The 95% methanol extract was isolated and purified by various chromatographic methods, and its structure was identified by modern spectroscopy and physicochemical properties. The in vitro inhibitory activities of the compounds on human cervical cancer cell line Hela and human gastric cancer cell line HGC-27 were detected by MTT method. Results Sixteen compounds were isolated and identified as isosclerone(1), engelhardione(2), 5-dehydroxy-octahydro-demethoxy curcumin-A(3),(E)-3,3′-dimethoxy-4,4′-dihydr-oxystilbene(4), blumenol A(5),(+)-erythro-7-O-ethylguaiacylglycerol(6), threo-3-(4-hydroxy-3,5-dimethoxyphenyl)-3-ethoxypropane-1,2-diol(7),(6R,9R)-blumenol B(8), hexahydro-demethoxycurcumin-A(9)、blumenol C(10),ethyl 3,4-dihydroxybenzoate(11),(+)-isolariciresinol(12), juglanin B(13), heptanoid(14), 4,17-dimethoxy-2-oxatricyclo [13.2.2.13,7] eicosa-3,5,7(20),15,17,18-hexaene-10,16-diol(15) and alternariol 9-methyl ether(16). Conclusion All compounds are isolated from Diaphragma Juglandis Fructus for the first time. Compounds 9 and 13 show inhibitory effect on Hela cells with IC50 of 25.33 and 16.29 μmol/L, respectively. Compound 13 shows certain activity on HGC-27 cells.

关 键 词：分心木 (+)-异落叶松脂醇 胡桃宁B hexahydro-demethoxycurcumin-A blumenol C 

分 类 号：R284.1[医药卫生—中药学]