基于UPLC-Q-TOF-MS/MS技术的益气健脾颗粒化学成分分析  

Identification of chemical constituents in Yiqi Jianpi Granules by UPLC-Q-TOF-MS/MS

作　　者：朱赟斐 谭善忠[3] 王洪兰[1,2] 单晨啸 崔小兵[1] 井山林[1] 李俊松[1,2] ZHU Yun-fei;TAN Shan-zhong;WANG Hong-lan;SHAN Chen-xiao;CUI Xiao-bing;JING Shan-lin;LI Jun-song(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Engineering Research Center for Efficient Delivery System of TCM,Nanjing 210023,China;Department of Integrated Traditional Chinese and Western Medicine,Nanjing Second Hospital&Nanjing Hospital Affiliated to Nanjing University of Chinese Medicine,Nanjing 210003,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药高效给药系统工程技术研究中心,江苏南京210023 [3]南京中医药大学附属南京医院,南京市第二医院中西医结合科,江苏南京210003

出　　处：《中草药》2022年第12期3601-3613,共13页Chinese Traditional and Herbal Drugs

基　　金：江苏省中医药领军人才项目(苏中医科教[2018]4号)。

摘　　要：目的 采用超高效液相色谱串联四级杆飞行时间质谱联用技术对益气健脾颗粒中化学成分进行初步鉴定和归属分类。方法 采用YMC-Pack ODS-A色谱柱(250 mm×4.6 mm,5μm),以0.2%甲酸水-甲醇为流动相体系,梯度洗脱,进样量8μL,体积流量1.0m L/min,在正、负离子模式下对色谱流出物进行质谱检测。结果 通过与对照品比对、自建数据库匹配,结合文献报道,从益气健脾颗粒中鉴定出130个化学成分,包括54个黄酮类、22个萜类、20个含氮化合物、14个香豆素类、12个有机酸类,还有8个醇苷类、鞘脂类等其他成分。结论 建立的定性分析方法能系统、快速地对益气健脾颗粒中的成分进行识别,为益气健脾颗粒的质量控制和研究药效物质基础提供重要依据。Objective To preliminarily identify and classify the chemical constituents in Yiqi Jianpi Granules(益气健脾颗粒)by ultra performance liquid chromatography quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS/MS).Methods The sample was performed on a YMC-Pack ODS-A chromatographic column(250 mm×4.6 mm,5μm)with 0.2%formic acid water-methanol as the mobile phase system by gradient elution.The injection volume was 8μL and the volume flow rate was 1.0 mL/min.The chromatographic effluents were detected by mass spectrometry in positive and negative ion mode.Results A total of 130 chemical components were identified by the methods of comparing with reference substances,matching self-built database,and literature reports,including 54 flavonoids,22 terpenoids,20 nitrogen-containing compounds,14 coumarins,12 organic acids,and eight glycosides,sphingolipids and other components.Conclusion The established method can systematically and quickly identify and analyze the chemical constituents of Yiqi Jianpi Granules,which could provide an important basis for the quality control and pharmacodynamic material basis study of Yiqi Jianpi Granules.

关 键 词：益气健脾颗粒 UPLC-Q-TOF-MS/MS 裂解规律 黄酮类 萜类 香豆素类 有机酸类 

分 类 号：R284.1[医药卫生—中药学]