女贞子对去卵巢小鼠脂肪生成的抑制作用及Wnt/β-catenin信号通路的影响  

Effect of Ligustri Lucidi Fructus on inhibiting adipogenesis and regulating Wnt/β-catenin signaling pathway in ovariectomized mice

作　　者：刘亚鸽 戴璇 刘田园 王钐 张悦怡 卢美希 陈贝贝 朱如愿 王丽丽 张东伟 LIU Ya-ge;DAI Xuan;LIU Tian-yuan;WANG Shan;ZHANG Yue-yi;LU Mei-xi;CHEN Bei-bei;ZHU Ru-yuan;WANG Li-li;ZHANG Dong-wei(Diabetes Research Center,School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学中医学院糖尿病研究中心,北京100029

出　　处：《中草药》2022年第12期3680-3686,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81874373);国家自然科学基金资助项目(82074235)。

摘　　要：目的 研究女贞子对去卵巢小鼠脂代谢的影响及其可能的作用机制。方法 采用切除双侧卵巢法建立小鼠骨质疏松模型,以女贞子水提液(3.5 g/kg)或戊酸雌二醇(0.1 mg/kg)连续干预去卵巢小鼠12周。采用苏木素-伊红(hematoxylin and eosin,HE)染色法观察各组小鼠内脏脂肪组织的病理变化;利用生化法测定血清中高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、总胆固醇(total cholesterol,TC)和三酰甘油(triglyceride,TG)水平;采用Western blotting法检测内脏脂肪组织中无翅型MMTV整合位点家族/β-连环蛋白(wingless type MMTV integration site family/β-catenin,Wnt/β-catenin)信号通路和脂肪生成相关蛋白的表达。结果 女贞子水提液能够显著抑制去卵巢小鼠体质量和体脂相对比率的增加(P<0.05),降低血清中TC、TG和LDL-C水平(P<0.05),减轻内脏脂肪组织细胞肥大(P<0.05)。同时,女贞子水提液可明显提高去卵巢小鼠内脏脂肪组织中Wnt 10b蛋白表达水平(P<0.05),降低p-β-catenin/β-catenin、过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptor γ,PPARγ)和CCAAT/增强子结合蛋白α(CCAAT/enhancer binding proteins α,C/EBPα)蛋白表达水平(P<0.05)。结论 女贞子水提液能够抑制去卵巢小鼠的脂肪生成,改善脂代谢,其作用机制可能与调控Wnt/β-catenin信号通路有关。Objective To study the effect and possible mechanism of Nvzhenzi(Ligustri Lucidi Fructus) on lipid metabolism in ovariectomized mice. Methods Osteoporotic mice model was established by removing the bilateral ovaries, and ovariectomized mice were ig Ligustri Lucidi Fructus aqueous extract(3.5 g/kg) or estradiol valerate(0.1 mg/kg) for 12 weeks. Pathological changes of visceral adipose tissue were observed by hematoxylin and eosin(HE) staining. Levels of high density lipoprotein cholesterol(HDLC), low density lipoprotein cholesterol(LDL-C), total cholesterol(TC) and triglyceride(TG) in serum were determined by biochemical method. Expressions of proteins involved in wingless type MMTV integration site family/β-catenin(Wnt/β-catenin) signaling pathway and its regulated adipogenesis-associated proteins in visceral adipose tissue were detected by Western blotting. Results Ligustri Lucidi Fructus aqueous extract significantly inhibited the increase of body weight and body fat relative ratio(P < 0.05), decreased the levels of TC, TG and LDL-C in serum(P < 0.05), and reduced cell hypertrophy of visceral adipose tissue in ovariectomized mice(P <0.05). Moreover, Ligustri Lucidi Fructus aqueous extract obviously increased the expression level of Wnt 10b(P < 0.05), decreased the expression levels of p-β-catenin/β-catenin, peroxisome proliferator-activated receptor γ(PPARγ) and CCAAT/enhancer binding proteins α(C/EBPα) in visceral adipose tissue of ovariectoized mice(P < 0.05). Conclusion Ligustri Lucidi Fructus aqueous extract could inhibit adipogenesis and further improve lipid metabolism in ovariectomized mice, which may be associated with the regulation of Wnt/β-catenin signaling pathway.

关 键 词：女贞子 去卵巢小鼠 脂肪代谢 WNT/Β-CATENIN信号通路 过氧化物酶体增殖物激活受体Γ CCAAT/增强子结合蛋白α 

分 类 号：R285.5[医药卫生—中药学]