c-型钠钛对小鼠胚胎体外发育的影响  

The effects of c-Type Natriuretic Peptide on development in vitro of mouse embryos

作　　者：杨诗雨 施泓如 王晓东 刘瑞妍 倪志翔 佟英伟 刘成 李翔 何长久 YANG Shiyu;SHI Hongru;WANG Xiaodong;LIU Ruiyan;NI Zhixiang;TONG Yingwei;LIU Cheng;Li Xiang;HE Changjiu(College of Veterinary Medicine Collge of Animal Sciences&Technology Huazhong Agriculture University/Key Laboratory of Agriculural Animal Genetics,Breeding and Reproduction of M inistry of Education/National Center for International Rescarch,Wuhan 430070,China)

机构地区：[1]华中农业大学动物科技学院动物医学院/农业动物遗传育种与繁殖教育部重点实验室/国家级国际联合研究中心,武汉430070

出　　处：《黑龙江动物繁殖》2022年第3期11-17,共7页Heilongjiang journal of animal reproduction

基　　金：湖北省农业科技创新中心畜禽遗传改良及健康养殖技术团队项目(201962000000130);湖北省大学生创新创业训练计划项目(202010504008)。

摘　　要：为了探讨c-型钠钛(CNP)对小鼠2细胞/4细胞胚胎转变及囊胚腔形成的影响,收集了小鼠原核期胚胎进行体外培养,随后在不同发育时期的培养体系中添加0,100,200μg/L CNP,分别为对照组、1组和2组。当胚胎发育至2-细胞阶段添加CNP,统计4细胞率和囊胚率;当胚胎发育至致密桑葚胚阶段添加CNP,统计囊胚成腔率、囊胚腔扩张率及囊胚孵化率,并测定扩张囊胚直径、囊胚腔面积占比及囊胚细胞数。结果表明:(1)2细胞阶段添加CNP后,与对照组相比,2组的4细胞发育率和囊胚率显著降低(P<0.05);(2)致密桑葚胚阶段添加CNP,与对照组相比,1组的囊胚成腔率、囊胚腔扩张率、囊胚腔直径、囊胚腔面积占比及孵化率无显著变化(P>0.05),但囊胚总细胞数显著降低(P<0.05);(3)致密桑葚胚阶段添加CNP,与对照组相比,2组的囊胚成腔率、囊胚腔扩张率、孵化率、囊胚腔直径、囊胚腔面积占及囊胚总细胞数均显著降低(P<0.05)。说明外源添加CNP抑制了小鼠2细胞/4细胞胚胎的转变、囊胚腔的形成与扩张,并降低了囊胚细胞数。In order to investigate the effects of c-Type Natriuretic Peptide(CNP) on the transition from 2-cell to 4-cell and blastocyst cavity formation in mice, the mouse prokaryotic embryos were collected and divided into 3 groups for in vitro culture, which were control group, group 1 and group 2. The doses of 0, 100 μg/L and 200 μg/L of CNP were added to control group, group 1 and group 2, respectively, at different developmental stages of embryos. When 2-cell embryos were developed, adding CNP to the culture mediums for observation of the 4-cell rate and blastocyst rate. When compacted morula were formed, adding CNP to the culture mediums for observation of blastocyst cavity formation rate, blastocyst expansion rate and blastocyst hatching rate, as well as the diameter of expanded blastocyst, the ratio of blastocyst area and the number of blastocyst cells. The results suggested:(1) With the addition of CNP at the 2-cell stage, the 4-cell rate and blastocyst rate of the group 2 were significantly reduced compared with the control group(P<0.05).(2) The addition of CNP at the stage of compacted morula did not affect the blastocyst cavity formation rate, the expansion rate of blastocyst, the diameter of blastocyst, the ratio of blastocyst area and the hatching rate in group 1(P>0.05). However, the CNP addition significantly decreased the total cell number of blastocysts, compared with the control group(P<0.05).(3) CNP addition at the stage of compacted morula significantly reduced the blastocyst cavity formation rate, the rate of blastocyst expansion, the hatching rate, the diameter of blastocyst, the ratio of blastocyst area and the number of blastocyst cells in group 2, compared with the control group(P<0.05). It can be deduced that exogenous addition of CNP could inhibit the transition of embryos from 2-cell to 4-cell in mice, blastocyst cavity formation and expansion, and reduced blastocyst cell number.

关 键 词：c-型钠肽 小鼠胚胎 体外发育 囊胚腔 2细胞 4细胞 囊胚细胞数 

分 类 号：Q813.7[生物学—生物工程]