基于ddPCR检测荷斯坦牛自由马丁现象的研究  

Research on detection of Holstein freemartinism phenomenon based on ddPCR

作　　者：王玉娇 李彦芹[1] 张汝美[1] 蔡高占 赵秀新 顾祥国 李俊婷 姚文龙 李建斌[1] 仲跻峰[1] WANG Yujiao;LI Yanqin;ZHANG Rumei;CAI Gaozhan;ZHAO Xiuxin;GU Xiangguo;LI Junting;YAO Wenlong;LI Jianbin;ZHONG Jifeng(Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agriculural Sciences,Jinan 250100,China;Shandong 0X Livestock Breeding Co.,Ltd.,Jinan 250108,China;Ningbo Milk Group Co.,Ltd.,Ningbo 315033,China;Ningbo Yinzhou Zhanqi Yingshan Cattle Farm,Ningbo 315145,China)

机构地区：[1]山东省农业科学院畜牧兽医研究所,济南250100 [2]山东奥克斯畜牧种业有限公司,济南250108 [3]宁波市牛奶集团有限公司,浙江宁波315033 [4]宁波市鄞州瞻岐鹰山牛场,浙江宁波315145

出　　处：《黑龙江动物繁殖》2022年第3期18-22,共5页Heilongjiang journal of animal reproduction

基　　金：山东省自然科学基金面上项目(ZR2021MC070);财政部和农业农村部国家现代农业产业技术体系项目(CARS-36);北沙牛遗传物质保存与人工授精技术应用示范项目(2019AS0010);奶牛高产核心群及β酪蛋白A2型高端品系选育与示范项目(2019B10018)。

摘　　要：为了鉴定异性双胎牛性器官发育紊乱疾病——牛自由马丁(freemartinism)综合征,试验选择7头中国荷斯坦异性孪生双胎的母牛进行微滴数字PCR(droplet digital PCR,ddPCR)检测,并根据异性孪生母犊不孕的个体染色体为XX/XY嵌合、正常母牛染色体为XX来判定是否为嵌合体。结果表明:此7个样本均存在XX/XY染色体嵌合,均为异性孪生不孕母犊。说明ddPCR方法用于鉴定牛自由马丁现象是有效的。To identify bovine freemartin syndrome, a disease caused by disorder of sexual organ development in co-twin heterosexual bovines, the peripheral bloods of seven Chinese Holstein freemartin cows were used to detect the existence of the XX/XY chimera by the way of droplet digital PCR(ddPCR). The determination references was that the karyotypes of infertile twin calves and normal cows should be XX/XY chimera and XX, respectively. The results showed that all seven samples were suffering XX/XY chimera and were hetero-twin infertile, and the ddPCR could be an effective way of identifying the bovine freemartinism.

关 键 词：荷斯坦牛 自由马丁 微滴数字PCR(ddPCR) 外周血 嵌合体 

分 类 号：S823.3[农业科学—畜牧学]