不同解冻方法对猪冷冻精液品质的影响  

Effects of thawing methods on the post-thawing quality of swine frozen semen

作　　者：郝爱玲 曲云淼 张祺 李孝娟 金金 潘丽娜 赵一丹 于达恒 张宇 HAO Ailing;QU Yunmiao;ZHANG Qi;LI Xiaojuan;JIN Jin;PAN Lina;ZHAO Yidan;YU Daheng;ZHANG Yu(Heilongjiang Animal Husbandry Service,Harbin 150069,China;Guangdong Shanwei Academy of Agricultural Sciences,Shanwei 516600,China)

机构地区：[1]黑龙江省畜牧总站,哈尔滨150069 [2]广东省汕尾市农业科学院,广东汕尾516600

出　　处：《黑龙江动物繁殖》2022年第3期42-45,共4页Heilongjiang journal of animal reproduction

摘　　要：为优化猪冷冻精液解冻方法、提高精液利用效率,试验采用两种不同的解冻方法解冻0.5 mL猪细管冷冻精液,一种在38℃水浴直接解冻30 s(1组),另一种在50℃水浴解冻16 s后再放到30℃水浴中静置40 s(2组)。对解冻后的精液分别进行精子活力、生存时间、顶体完整率的测定和顶体形态观察。结果表明:2组解冻精液的精子活力、顶体完整率极显著高于1组(P<0.01),但两组的生存时间差异不显著(P>0.05);精子顶体形态学观察发现,两组冻精的精子顶体膨胀、缺损情况都较严重。说明与38℃慢速解冻冷冻精液相比,50℃快速解冻的猪细管冷冻精液的精子活力好而且顶体完整率高。In order to optimize the thawing methods and improve the post-thawing quality of swine frozen semen, 0.5 mL straws were thawed with two thawing methods, which were the way of thawing either in a water bath either at 38 ℃ for 30 s(group 1)thawing or in the water bath at 50 ℃ for 16 s and then thawing in the water bath at 30 ℃ for 40 s(group 2). The sperm viability, survival time and acrosome integrity rate of the thawed semen were measured and acrosome morphology was observed.The results showed that the sperm viability and acrosome integrity rate in group 2 were significantly higher(P<0.01) than those in group 1. However, no significant difference was found on survival time between the two thawing methods(P>0.05). Moreover, the swelling or defects in acrosomes were found in thawed semen. It indicated that the sperm motility and acrosome integrity rate of the frozen pig semen from straws thawed rapidly at 50 ℃ were better than those thawed slowly at 38 ℃.

关 键 词：猪冷冻精液 解冻方法 精子活力 生存时间 顶体完整率 

分 类 号：S828.34[农业科学—畜牧学]