An in planta haploid induction system in Brassica napus  

作　　者：Yifan Li Dan Li Qing Xiao Huadong Wang Jing Wen Jinxing Tu Jinxiong Shen Tingdong Fu Bin Yi 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement,National Center of Rapeseed Improvement,Huazhong Agricultural University,Wuhan 430070,China [2]Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Jiangxi Agricultural University,Nanchang 330045,China

出　　处：《Journal of Integrative Plant Biology》2022年第6期1140-1144,共5页植物学报（英文版）

摘　　要：Doubled haploid technology is widely used to accelerate plant breeding,but its use in the important oilseed crop Brassica napus L.is limited because B.napus haploids could only be obtained through in vitro anther or microspore cultures.Recently,maize(Zea mays)lines containing mutations in Domain of unknown function 679 membrane protein(DMP)were used as haploid inducer lines.This new haploid induction mechanism has been extended to several other plants,including the dicots Arabidopsis thaliana,tomato(Solanum lycopersicum),and tobacco(Nicotiana tabacum).Here,we knocked out four BnaDMP genes in the B.napus cultivar Westar using a clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 vector with an enhanced green fluorescent protein expression cassette.Plants with DMP mutations in B.napus in the T_(0),T_(1),and T_(2) generations exhibited a haploid induction rate up to 2.53%.These results suggest that targeting BnaDMP could be useful for haploid induction in B.napus.

关 键 词：Brassica napus CRISPR/Cas9 fluorescence screening maternal haploid induction 

分 类 号：S565.4[农业科学—作物学]