尪痹胶囊抗炎镇痛作用及其机制研究  

Study on the anti-inflammatory and analgesic effects of WangBi JiaoNang and its mechanism

作　　者：刘婷婷 张雨萌 刘峻男 赵旻[1] 王淼[2] 赵春杰[1] LIU Tingting;ZHANG Yumeng;LIU Junnan;ZHAO Min;WANG Miao;ZHAO Chunjie(School of Pharmacy,Shenyang Pharmaceutical University,Wenhua Road 103,Shenyang110016,Liaoning Province,China;School of Life Science and Biopharmaceutics,Shenyang Pharmaceutical University,Wenhua Road 103,Shenyang110016,Liaoning Province,China)

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2022年第5期556-562,共7页Journal of Shenyang Pharmaceutical University

摘　　要：目的考察尪痹胶囊抗炎镇痛的作用效果及其作用机制。方法(1)60只KM系小鼠,随机分为空白组、模型组、阿司匹林组、尪痹胶囊(高、中、低)给药组,每组10只,连续给药7 d,末次给药0.5 h后建立二甲苯诱导的小鼠耳肿胀模型,计算小鼠耳肿胀度、肿胀抑制率,HE染色法观察致炎侧小鼠耳组织病理学变化,ELISA法测定小鼠血清中TNF-α、IL-6的含量。(2)60只KM系小鼠,按抗炎实验进行分组给药,于末次给药0.5 h后建立醋酸扭体致痛模型,计算各组小鼠疼痛反应潜伏期、20 min内扭体次数,ELISA法测定小鼠血清、大脑皮质中的β-EP和血清中的PGE2。结果(1)与模型组相比,尪痹胶囊高剂量组和中剂量组能够显著抑制二甲苯诱导的小鼠耳肿胀;各给药组能明显改善耳组织的炎症反应;各给药组均能显著下调耳肿胀小鼠血清中的TNF-α和IL-6水平。(2)与模型组相比,尪痹胶囊高剂量组和中剂量组能够明显抑制由醋酸引起的小鼠扭体反应;高剂量组和中剂量组能够明显延长小鼠扭体反应的潜伏期;各给药组均能显著升高小鼠血清和大脑皮质中的β-EP含量,降低血清中的PGE2含量。结论尪痹胶囊对二甲苯诱导的小鼠耳肿胀致炎模型具有良好的抑制作用,能够减轻由醋酸引起的小鼠疼痛反应,证明该药具有良好的抗炎镇痛作用,其作用发挥的机制可能与降低小鼠血清中的TNF-α、IL-6和PGE2,升高血清和大脑皮质中的β-EP有关。Objective To investigate the anti-inflammatory and analgesic effect and mechanism of Wangbi JiaoNang.Methods(1)60 KM mice were randomly divided into blank group,model group,aspirin group and Wangbi JiaoNang(high,medium and low)administration group,with 10 mice in each group.After continuous administration for 7 days,the mice ear swelling model induced by xylene was established 0.5 h after the last administration.Mice ear swelling degree,swelling inhibition rate,the histopathological changes and the content of TNF-αand IL-6 were used to evaluate the anti-inflammatory effect of Wangbi JiaoNang.(2)60 km mice were divided into groups according to the anti-inflammatory experiment.The pain model induced by acetic acid was established 0.5 h after the last administration.The latency of pain response,number of writhing within 20 minutes,and the changes ofβ-EP in serum and cerebral cortex and PGE2 in serum in each group were used to evaluate the analgesic effect of Wangbi JiaoNang.Results(1)Compared with the model group,the high-dose and medium-dose groups of Wangbi JiaoNang could significantly inhibit the ear swelling induced by xylene;Each administration group could significantly improve the inflammatory response of ear tissue;All administration groups could significantly down-regulate TNF-αand IL-6 in the serum of the swelling of mouse ear.(2)Compared with the model group,the high-dose and medium-dose groups of Wangbi JiaoNang could significantly inhibit the writhing reaction and prolong the latency of writhing reaction of mice caused by acetic acid;All administration groups could significantly increase the levels ofβ-EP content in serum and cerebral cortex and reduce the content of PGE2 in serum.Conclusion Wangbi JiaoNang has a good inhibitory effect on the inflammation induced by xylene-induced ear swelling in mice,and can reduce the pain response induced by acetic acid in mice,which proves that it has good anti-inflammatory and analgesic effects.The mechanism of its action may be related to the decrease of TNF-α

关 键 词：尪痹胶囊 抗炎镇痛 醋酸扭体 小鼠耳肿胀 炎症因子 

分 类 号：R917[医药卫生—药物分析学]