MsxC基因在斑马鱼肌间刺和大侧肌发育中的作用  

Role of MsxC gene in the development of intermuscular bones and axial muscle in zebrafish

作　　者：庞天抒 车金远 范纯新[1,2] 鲍宝龙[1,2] PANG Tianshu;CHE Jinyuan;FAN Chunxin;BAO Baolong(Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Center for Experimental Fisheries Science Education,,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学水产种质资源发掘与利用教育部重点实验室,上海201306 [2]上海海洋大学水产科学国家级实验教学示范中心,上海201306

出　　处：《上海海洋大学学报》2022年第2期328-335,共8页Journal of Shanghai Ocean University

基　　金：国家自然科学基金面上项目(31872546)。

摘　　要：肌间刺存在于有些真骨鱼类的肌间隔中,作为Hox基因家族成员,MsxC(muscle segment homeobox C)基因的表达模式与唇䱻肌间刺形成有一定的相关性。为了进一步探究MsxC基因在肌间刺形成中的作用,利用CRISPR/Cas9基因编辑技术,建立斑马鱼MsxC基因纯合突变体(MsxC^(^(-/-)))。结果表明:与野生型斑马鱼相比,MsxC^(-/-)斑马鱼尾部的髓弓小骨和脉弓小骨平均缩短15%以上,差异极显著,而躯干部的肌间刺长度则无明显差异;MsxC^(-/-)斑马鱼大侧肌肌隔周围白肌细胞分布疏松,且排列混乱,越靠近尾部差异越明显;MsxC^(-/-)突变体红肌面积增大,越靠近尾部,红肌面积增大越明显,尾部红肌面积增大2.22%(P<0.01),而躯干靠前部位面积增大0.97%(P<0.05)。这表明,MsxC基因的缺失既影响了肌间刺的形成,同时也影响了大侧肌的发育。Intermuscular bones(IBs)exist in the intermuscular septums of some species of teleosts.MsxC(muscle segment homeobox C)gene,a member of Hox gene family,was postulated to potentially play a role during the development of intermuscular bone in Hemibarbus labeo.In this study,we deleted MsxC gene in zebrafish genome by CRISPR/Cas9 technology to create the MsxC;strain.Compared with wild-type zebrafish,MsxC ^(-/-)zebrafish had shorter IBs in rear part of body,where both epineurals and epipleurals were shortened by more than 15%on average.However,there was no significant difference in the length of IBs in the anterior part of trunk.We also found less fast muscle fibers nearby septum in MsxC ^(-/-) zebrafish,especially in rear part of body.On the contrary,more slow muscle fibers were found in MsxC^(-/-) zebrafish.Compared with wild type,the area of slow muscle in rear part of MsxC^(-/-) zebrafish was increased by 2.22%(P<0.01),whereas the area of trunk anterior part was increased by 0.97%(P<0.05).All these results suggested that the deletion of MsxC gene affected both the formation of IBs and the development of axial muscle.

关 键 词：斑马鱼 MsxC 肌间刺 大侧肌 CRISPR/Cas9 

分 类 号：S917[农业科学—水产科学]