我国2015-2017年急性乙型肝炎病例HBV基因型分布特征分析  

Epidemiological distribution of hepatitis B virus genotypes in acute hepatitis B cases in China,2015-2017

作　　者：张楠 张爽[1] 王锋[1] 尹遵栋[2] 苏秋东[1] 张国民[2] 缪宁[2] 毕胜利[1] 王富珍[2] 沈立萍[1] Zhang Nan;Zhang Shuang;Wang Feng;Yin Zundong;Su Qiudong;Zhang Guomin;Miao Ning;Bi Shengli;Wang Fuzhen;Shen Liping(National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Department of National Immunization Program,Chinese Center for Disease Control and Prevention,Beijing 100050,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京102206 [2]中国疾病预防控制中心免疫规划中心,北京100050

出　　处：《中华流行病学杂志》2022年第6期865-870,共6页Chinese Journal of Epidemiology

基　　金：国家科技重大专项(2017ZX10105015-001-002)。

摘　　要：目的分析我国急性乙型肝炎(乙肝)病例HBV基因型流行病学分布特征。方法收集2015-2017年中国疾病预防控制信息系统报告的急性乙肝病例620例,通过巢式PCR扩增获得全长HBV基因组,构建系统发育树确定HBV基因型,结合基础资料分析HBV基因型的分布情况。结果在620例急性乙肝病例中,成功分型519例(83.71%,519/620),包括A型(0.19%,1/519)、B型(27.17%,141/519)、C型(62.04%,322/519)、D型(9.06%,47/519)、I型(0.77%,4/519)和CD重组型(0.77%,4/519);2个主要的基因亚型为B2(95.03%,134/141)和C2(72.67%,234/322)。基因型在我国7个地区分布不同,C型在东北(94.55%,52/55)、华北(93.85%,61/65)、华东(78.87%,56/71)和华南地区(58.14%,50/86)的比例较高,B型在华中(58.07%,36/62)和西南(52.94%,45/85)地区的比例较高,西北地区的D型(48.42%,46/95)比例较高;共有515例成功获得血清型,包括adr(57.48%,296/515)、adw(30.87%,159/515)、ayr(0.19%,1/515)、ayw(11.46%,59/515)。B型以adw血清型(92.14%,129/140)为主,C型以adr血清型(91.88%,294/320)为主,D型均为ayw血清型;不同性别、年龄组中,基因型分布差异无统计学意义(P>0.05)。结论2015-2017年我国急性乙肝基因型以B、C和D型为主,东北、华北、华东和华南地区C型为主;华中、西南地区B、C型均有,B型为主,D型多分布于西北地区;急性乙肝基因型与血清型存在相关性,B型以adw血清型为主,C型以adr血清型为主,D型均为ayw血清型;不同性别和年龄组中急性乙肝基因型分布无差异。Objective To analyze the genotype distribution of acute hepatitis B virus in China.Methods A total of six hundred and twenty acute Hepatitis B cases reported to China Information System for Diseases Control and Prevention from 2015 to 2017 were selected.First,the full-length HBV genome was obtained by nested PCR amplification.In addition,the HBV genotype was determined by constructing a phylogeny tree.Finally,using primarydata,HBV genotype distribution was analyzed.Results A total of 519(83.71%,519/620)sequences were obtained genotype of 620 acute hepatitis B cases,including A(0.19%,1/519),B(27.17%,141/519),C(62.04%,322/519),D(9.06%,47/519),I(0.77%,4/519)and C/D(0.77%,4/519);B2(95.03%,134/141)and C2(72.67%,234/322)were the two major subgenotypes.Genotypes were distributed differently in seven regions of China.The proportion of genotype C appeared higher in Northeast China(94.55%,52/55),North China(93.85%,61/65),East China(78.87%,56/71),and South China(58.14%,50/86).The proportion of genotype B was higher in Central China(58.07%,36/62)and Southwest China(52.94%,45/85),the proportion of genotype D was the highest in Northwest China(48.42%,46/95).A total of 515 cases were classified as serotypes,including'adr'(57.48%,296/515),'adw'(30.87%,159/515),'ayr'(0.19%,1/515),and'ayw'(11.46%,59/515).Genotype B was dominated by'adw'serotype(92.14%,129/140),genotype C was dominated by'adr'serotype(91.88%,294/320),all genotype D were'ayw'serotype.The genotype of acute hepatitis B was correlated with serotype,'adw'was dominant in genotype B,'adr'was dominant in genotype C and'ayw'was dominant in genotype D.In different gender and age group,there was no statistical significance ingenotype distribution(P>0.05).Conclusions The genotype of acute hepatitis B in China from 2015 to 2017 was mainly B,C,and D;genotype C was dominant in the Northeast China,North China,East China and South China;B and C were common in Central and Southwest China,and genotype B was dominant.Genotype D was primarily distributed in Northwest China.The genotype

关 键 词：急性乙型肝炎 乙型肝炎病毒 基因型 分子流行病学 

分 类 号：R512.62[医药卫生—内科学]