L-瓜氨酸对缺血再灌注损伤心肌的保护作用及机制研究  

Protective effect and mechanism of L-citrulline on myocardial ischemia-reperfusion injury

作　　者：李志乐[1] 韦秋宇 言纬 刘燕[1] 韦宝敏[1] 黄照河[1] LI Zhile;WEI Qiuyu;YAN Wei;LIU Yan;WEI Baomin;HUANG Zhaohe(Department of Cardiovascular Medicine,Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,China)

机构地区：[1]右江民族医学院附属医院心血管内科,百色533000

出　　处：《国际心血管病杂志》2022年第3期164-168,175,共6页International Journal of Cardiovascular Disease

基　　金：国家自然科学基金(81860797);右江民族医学院附属医院高层次人才科研项目(Y20196318)。

摘　　要：目的:探讨L-瓜氨酸对缺血再灌注损伤心肌的保护作用及机制。方法:选取健康新西兰大白兔30只,随机分为对照组、模型组和实验组,每组10只。构建MIRI模型,实验组予L-瓜氨酸100µg/L预处理,模型组给予等量生理盐水,对照组只开胸不结扎。收集各组血液和心肌组织标本,采用全自动生化分析仪检测血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)和乳酸脱氢酶(LDH)水平,苏木精-伊红(HE)染色观察心肌病理学改变,2,3,5-三苯基氯化四氮唑(TTC)染色检测心肌梗死面积,原位末端转移酶标记(TUNEL)染色检测心肌凋亡情况。结果:与对照组相比,模型组兔出现急性心肌损伤表现,HE染色显示心肌组织纤维细胞和肌节结构紊乱,细胞核固缩,血清CK、CK-MB、LDH水平均明显升高(P均<0.05),心肌梗死面积、心肌凋亡率均明显增加(P均<0.05)。与模型组相比,实验组心肌损伤减轻,血清CK、CK-MB、LDH水平降低(P均<0.05),心肌梗死面积及心肌凋亡率减少(P均<0.05)。结论:L-瓜氨酸通过抑制心肌细胞凋亡,发挥对缺血再灌注损伤的保护作用。Objectives:To investigate the protective effect and mechanism of L-citrulline on myocardial ischemia reperfusion injury(MIRI).Methods:A total of 30 healthy New Zealand white rabbits were randomly divided into sham operation group(control group),ischemia reperfusion group(model group)and model+L-citrulline group(experimental group),with 10 rabbits in each group.The MIRI model was established firstly,the experimental group was pretreated with L-citrulline 100µg/L.The model group was given the same amount of normal saline,and the control group was given only thoracotomy without ligation.At the end of the experiment,blood and heart tissue samples were collected for the subsequent experiments.The serum levels of CK,CK-MB and LDH were detected by automatic biochemical analyzer,and the pathological changes of myocardiial tissue were observed by HE staining.TTC staining and TUNEL staining were used to observe the myocardial infarct size and myocardial apoptosis.Results:Compared with the control group,acute myocardial injury was observed in model group.HE staining showed structural disorder of fibrocytes,sarcomere and nuclear pyknosis.The serum levels of CK,CK-MB and LDH,and myocardial infarct area and apoptosis rate were significantly increased in the model group(all P<0.05).Compared with the model group,myocardial injury in the experimental group could be reduced significantly by exogenous citrulline.The serum levels of CK,CK-MB and LDH,and myocardial infarct area and apoptosis rate were significantly decreased in experimental group(all P<0.05).Conclusion:L-citrulline plays a protective role on MIRI by inhibiting myocardial apoptosis.

关 键 词：L-瓜氨酸 心肌缺血再灌注损伤 心肌细胞凋亡 心肌梗死 

分 类 号：R285.5[医药卫生—中药学]