XAD-7HP树脂纯化款冬花多糖工艺研究  

Study on Purification of Polysaccharide from Farfarae Flos by MAR of XAD-7HP

作　　者：韩毅丽 张澍 裴晓丽 HAN Yi-li;ZHANG Shu;PEI Xiao-li(College of Chinese Medicine and Food Engineering,Shanxi University of Chinese Medicine,Shanxi Jinzhong 030619,China)

机构地区：[1]山西中医药大学中药与食品工程学院,山西晋中030619

出　　处：《广州化工》2022年第12期60-62,共3页GuangZhou Chemical Industry

基　　金：山西中医药大学科技创新能力培育项目(No:2019PY-145);山西省中医药管理局科研项目(No:2020ZYYC006)。

摘　　要：研究XAD-7HP型大孔吸附树脂纯化款冬粗多糖的影响因素。采用单因素分析法考察了影响款冬花多糖纯化效果的主要因素款冬花多糖的上样浓度、上样速度、乙醇洗脱液浓度、洗脱速度四个因素。结果表明款冬花多糖的最佳纯化工艺为:0.3208 mg/mL的款冬花多糖液以1.0 mL/min的速度上样,采用65%的乙醇溶液以1.0 mL/min的流速进行洗脱。经XAD-7HP树脂纯化后,款冬花多糖的纯度达到了72.98%,纯化效果显著。The influencing factors of XAD-7HP MACROPROUS ADSORPTION RESIN for purification of polysaccharide from Farfarae Flos were studied.The main factors affecting the purification effect of polysaccharide from Farfarae Flos of the sample concentration,the sample rate,the ethanol eluent concentration and the elution speed on the purification effect of polysaccharides were investigated by single factor analysis.The results showed that the optimum purtication process of polysaccharide polysaccharide from Farfarae Flos was as follow:the sample concentration was 0.3208 mg/mL,the sample rate was 1.0 mL/min,the ethanol eluent concentration was 65%and the elution speed was 1.0 mL/min.The content of coltsfoot polysaccharides increased to 72.98%,the purification effect was significant.

关 键 词：款冬花 多糖 XAD-7HP大孔吸附树脂 纯化 

分 类 号：O652.6[理学—分析化学]