分光光度法测定地表水中叶绿素a的进一步研究  

Further Study on Determination of Chlorophyll a in Surface Water by Spectrophotometry

作　　者：王一会 王志瑞 吴德景 郭莹 WANG Yi-hui;WANG Zhi-rui;WU De-jing;GUO Ying(Anshun Ecological Environment Monitoring Center,Guizhou Anshun 561000,China)

机构地区：[1]安顺生态环境监测中心,贵州安顺561000

出　　处：《广州化工》2022年第12期103-105,共3页GuangZhou Chemical Industry

摘　　要：本研究结合已有权威标准和相关报道,以安顺市饮用水源点的地表水为供试对象,探索并优化分光光度法测定地表水中叶绿素a的条件。研究主要通过改进叶绿素a的提取条件和改变比色皿长度来进行实验。研究结果表明:通过改进叶绿素a的提取条件和改变比色皿长度,可有效减少叶绿素a在提取过程中的损失,降低检出限,更准确地反应地表水中叶绿素a的含量。On the basis of pre-existing authoritative standards and researches,as well as the surface water of Anshun as the test object,conditions for determinating the content of chlorophyll a in surface water were explored and optimized by spectrophotometric method.The experiments were carried out by improving the extraction conditions of chlorophyll a and changing the length of cuvette.Experimental results showed that it could effectively reduce the loss of chlorophyll a in the extraction process and reduce the detection limit by the means of optimizing the extraction conditions of chlorophyll a and changing the cuvette length,thus further indicate the content of chlorophyll a in surface water more accurately.

关 键 词：叶绿素A 分光光度法 提取条件 比色皿长度 地表水 

分 类 号：X832[环境科学与工程—环境工程]