香烟烟雾颗粒通过MAPK和NF-κB信号通路上调内皮素受体诱导大鼠肠系膜动脉平滑肌收缩  

Endothelin receptor-mediated vaso contraction via activation of MAPK/NF-κB pathways in the mesenteric artery of rats by cigarette smoking particle

作　　者：张盼 王钰莹 王婷 史永恒[1,2] 刘继平 徐仓宝[3] 胡锐 王川 ZHANG Pan;WANG Yu-ying;WANG Ting;SHI Yong-heng;LIU Ji-ping;XU Cang-bao;HU Rui;WANG Chuan(College of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang Shaanxi 712046;Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine,Shaanxi Bureau of Traditional Chinese Medicine,Xianyang Shaanxi 712046;Shaanxi Key Laboratory of Ischemic Cardiovascular Disease,Institute of Basic and Translational Medicine of Xi’an Medical University,Xi’an 710021)

机构地区：[1]陕西中医药大学药学院,陕西咸阳712046 [2]陕西省中医药管理局中药药效机制与物质基础重点研究室,陕西咸阳712046 [3]西安医学院基础与转化医学研究所/陕西省缺血性心血管疾病重点实验室,西安710021

出　　处：《中南药学》2022年第6期1238-1244,共7页Central South Pharmacy

基　　金：国家自然科学基金项目(No.81800401);大学生创新创业训练计划项目(No.S202010716021;No.S202010716024);陕西中医药大学学科创新团队(No.2019-YL13)。

摘　　要：目的研究香烟烟雾颗粒(DSP)对大鼠肠系膜动脉平滑肌内皮素受体的影响及作用机制。方法采用离体血管环技术观察DSP对内皮素受体介导的肠系膜动脉平滑肌收缩功能的影响,Western blot法检测内皮素受体以及MAPK和NF-κB信号通路相关蛋白的表达。结果DSP可以增强内皮素受体激动剂引起的血管收缩并使内皮素受体蛋白表达增加,ERK1/2和P38通路抑制剂可以抑制DSP诱导的血管收缩和降低内皮素受体的表达。结论DSP通过MAPK和NF-κB信号通路上调内皮素受体诱导大鼠肠系膜动脉平滑肌收缩。Objective To determine the effect of cigarette smoking particles on the vasoconstrictions induced by endothelin receptors in the mesenteric artery of rats.Methods In vitro aorta ring culture was used to observe how cigarette smoking particles affected vasoconstrictions induced by endothelin receptors and MAPK/NF-κB pathway related proteins expression by Western blot.Results Cigarette smoking particles enhanced mesenteric artery constriction induced by endothelin receptor agonists and increased the expression of endothelin receptors,while the inhibitors of ERK1/2 and P38 signaling pathway inhibited the vasoconstriction responses and decreased the protein expression of endothelin receptors.Conclusion Cigarette smoking particles can up-regulate endothelin receptor-mediated vaso contraction by activating MAPK and NF-κB signaling pathways in the mesenteric artery of rats.

关 键 词：香烟烟雾颗粒 大鼠肠系膜动脉 内皮素受体 MAPK信号通路 NF-ΚB信号通路 

分 类 号：R965.1[医药卫生—药理学]