山竹果皮中总氧杂蒽酮通过调控miR-338-3p抑制胃癌细胞AGS增殖、迁移侵袭及炎症反应的实验研究  

Total xanthone in mangosteen inhibits cell proliferation,migration,invasion,and inflammatory response in gastric cancer cells by regulating miR-338-3p

作　　者：王芬芬 王秀芳 胡剑浩 王银娟 Fen-Fen Wang;Xiu-Fang Wang;Jian-Hao Hu;Yin-Juan Wang(Department of Gastroenterology,Affiliated Hospital of Shaoxing University of Arts and Sciences,Shaoxing 312000,Zhejiang Province,China)

机构地区：[1]绍兴文理学院附属医院消化内科,浙江省绍兴市312000

出　　处：《世界华人消化杂志》2022年第13期579-586,共8页World Chinese Journal of Digestology

摘　　要：背景山竹果皮中总氧杂蒽酮具有抗肿瘤作用,但其对胃癌细胞生物学行为的影响尚未可知,miRNA在胃癌发生发展过程中可发挥重要调控作用,并可能作为胃癌治疗的潜在靶点,但其是否可作为山竹果皮中总氧杂蒽酮治疗胃癌的潜在靶点尚未可知.目的探讨山竹果皮中总氧杂蒽酮对胃癌细胞AGS生物学行为的影响及其可能作用机制.方法以人胃癌细胞AGS为研究对象,随机分组:NC组、低剂量组、中剂量组、高剂量组、miR-NC组、miR-338-3p组、高剂量组+anti-miR-NC组、高剂量组+anti-miR-338-3p组;MTT法、Transwell实验分别检测细胞增殖、迁移及侵袭;ELISA法检测肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素6(interleukin-6,IL-6)、白细胞介素-1β(interleukin-1β,IL-1β)的水平;qRT-PCR法检测miR-338-3p的表达量.结果山竹果皮中总氧杂蒽酮处理后细胞活力、TNF-α、IL-6、IL-1β水平降低(P<0.05),迁移及侵袭细胞数减少(P<0.05),miR-338-3p的表达量升高(P<0.05);转染miR-338-3p mimics后细胞活力、TNF-α、IL-6、IL-1β水平降低(P<0.05),迁移及侵袭细胞数减少(P<0.05);转染anti-miR-338-3p可逆转山竹果皮中总氧杂蒽酮对AGS细胞生物学行为的作用.结论山竹果皮中总氧杂蒽酮可通过促进miR-338-3p表达而抑制胃癌细胞增殖、迁移、侵袭及炎症反应.BACKGROUND Total xanthone in mangosteen has antitumor effect,but its effect on the biological behavior of gastric cancer cells is still unknown.MicroRNAs(miRNAs)can play an important regulatory role in the occurrence and development of gastric cancer and may be a potential target for gastric cancer treatment,but whether they could be potential targets for total xanthone in mangosteen in the treatment of gastric cancer is unknown.AIM To explore the effect of total xanthone in mangosteen on the biological behavior of gastric cancer AGS cells and the possible mechanism involved.METHODS Human gastric cancer AGS cells were randomly divided into the following groups:NC group,low-dose xanthone group,medium-dose xanthone group,high-dose xanthone group,miR-NC group,miR-338-3p group,high-dose xanthone+anti-miR-NC group,and high-dose xanthone group+anti-miR-338-3p group.MTT and Transwell assays were used to detect cell proliferation,migration,and invasion.ELISA was used to detect the levels of TNF-α,IL-6,and IL-1β.qRT-PCR was used to detect the expression of miR-338-3p.RESULTS After treatment with total xanthone in mangosteen,cell viability and the levels of TNF-α,IL-6,and IL-1βwere decreased(P<0.05),the number of migrating and invasive cells was decreased(P<0.05),and the expression of miR-338-3p was increased(P<0.05).After transfection with miR-338-3p mimic,cell viability and the levels of TNF-α,IL-6,and IL-1βwere decreased(P<0.05),and the number of migrating and invasive cells was decreased(P<0.05).Transfection with anti-miR-338-3p could reverse the effect of total xanthone in mangosteen on the biological behavior of AGS cells.CONCLUSION Total xanthone in mangosteen can inhibit the proliferation,migration,invasion,and inflammatory response of gastric cancer cells by promoting the expression of miR-338-3p.

关 键 词：胃癌 山竹 果皮 总氧杂蒽酮 miR-338-3p 细胞增殖 迁移侵袭 炎症 

分 类 号：R285[医药卫生—中药学]