痛风病人周围血中性粒细胞外诱捕网的测定及其意义  

Determination of peripheral blood neutrophil extracellular traps and its significance in patients with gout

作　　者：谭悦 刘童 陆继娣 余家静 王亚辉 李兴锐 TAN Yue;LIU Tong;LU Ji-di;YU Jia-jing;WANG Ya-hui;LI Xing-rui(Department of Rheumatology and Immunology,The Lu′an Hospital Affiliated to Anhui Medical University,The Lu′an People′s Hospital,Lu′an Anhui 237005,China)

机构地区：[1]安徽医科大学附属六安医院,六安市人民医院风湿免疫科,安徽六安237005

出　　处：《蚌埠医学院学报》2022年第6期747-750,共4页Journal of Bengbu Medical College

基　　金：安徽省六安市人民医院面上项目(2021kykt14);安徽省六安市人民医院青年项目(2021kykt30)。

摘　　要：目的:比较中性粒细胞外诱捕网(NETs)在痛风病人与健康对照组血清中表达水平的差异,以探讨NETs在痛风中可能存在的作用。方法:用MPO-DNA ELISA法进行间接定量检测40例痛风病人和24名健康对照者周围血血清中游离NETs水平并分析其差异,同时分析痛风病人周围血NETs水平与血清红细胞沉降率(ESR)、C反应蛋白(CRP)、尿酸、病程等指标的相关性,及使用秋水仙碱组(19例)和未使用秋水仙碱组(21例)痛风病人血清中NETs水平的差异。结果:痛风组NETs的浓度(2119.62±1843.36)pg/mL高于健康对照组(1894.66±113.52)pg/mL,差异有统计学意义(P<0.01)。痛风组血清NETs水平与ESR值呈正相关(P<0.05),与CRP值呈正相关(P<0.01),与血尿酸、病程等均无相关性(P>0.05)。未服用秋水仙碱组痛风病人血清NETs浓度(2138.24±173.60)pg/mL高于服用秋水仙碱组(2099.05±196.23)pg/mL,但差异无统计学意义(P>0.05)。结论:中性粒细胞可能通过一种新的死亡方式,即网捕死亡形成NETs,参与痛风性关节炎的发病,血清NETs水平可能与痛风疾病活动度有关。Objective:To compare the expression levels of neutrophil extracellular traps(NETs)between gout patients and healthy controls,and explore the possible role of NETs in gout.Methods:The serum levels of free NETs in 40 gout patients and 24 healthy controls were detected by MPO-DNA ELISA,and the association between serum NETs and ESR,CRP,uric acid,length of the course of the disease were analyzed.The difference of the serum level of NETs between the colchicine group(19 cases)and noncolchicine group(21 cases)was compared.Results:The serum levels of NETs in gout group[(2119.62±1843.36)pg/mL]were significantly higher than that in healthy control group[(1894.66±113.52)pg/mL](Ρ<0.01).The serum level of NETs in gout group was positively correlated with ESR and CRP(P<0.05 and P<0.01),but not correlated with uric acid and length of the course of the disease(P>0.05).The serum level of NETs in gout group treated without colchicine[(2138.24±173.60)pg/mL]was high compared with the gout group treated with colchicine[(2099.05±196.23)pg/mL],but the difference of which was not statistically significant(P>0.05).Conclusions:The neutrophils may be involved in the pathogenesis of gout arthritis by forming NETs in a new mode of death.The serum level of NETs may be related to the gout disease activity.

关 键 词：关节炎 痛风 中性粒细胞外诱捕网 

分 类 号：R589.7[医药卫生—内分泌]