中药制剂702糖浆薄层色谱鉴别与含量测定  

TLC identification and content determination of traditional Chinese medicine preparation 702 syrup

作　　者：何佩贞[1] 黄春喜[1] 何成章[1] 蔡丹昭[2] 罗捷[1] 罗育[2] He Peizhen;Huang Chunxi;He Chengzhang;Cai Danzhao;Luo Jie;Luo Yu(Department of Pharmacy,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China;School of Basic Medical Sciences,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学第一附属医院药学部,南宁530021 [2]广西医科大学基础医学院,南宁530021

出　　处：《广西医科大学学报》2022年第6期987-992,共6页Journal of Guangxi Medical University

基　　金：广西高校生物分子医学研究重点实验室开放课题(No.GXBMR201607);广西高校科学技术研究项目(No.KY2015LX058);广西医科大学青年科学基金项目(No.GXMUYSF2014015)。

摘　　要：目的:建立702糖浆中何首乌、鸡血藤、钩藤的薄层色谱鉴别及何首乌中有效成分2,3,5,4’-四羟基二苯乙烯-2-O-β-D葡萄糖苷(简称二苯乙烯苷)的含量测定方法,为702糖浆的质量控制提供依据。方法:采用薄层色谱法(TLC)定性鉴别702糖浆中的何首乌、鸡血藤、钩藤等成分,采用高效液相色谱法(HPLC)测定二苯乙烯苷的含量。结果:何首乌、鸡血藤、钩藤等的TLC斑点清晰、阴性对照无干扰,具有较强的特异性;二苯乙烯苷在1.0146~25.0190μg/mL范围内与峰面积呈现良好线性关系(r=0.9999),平均加样回收率为99.6%,RSD为1.58%;各批样本间二苯乙烯苷含量比较,差异无统计学意义(P>0.05)。结论:该方法可以定性鉴别702糖浆并测定其有效成分的含量,且操作简便,重复性好,稳定性高,可用于702糖浆的质量控制。Objective:To establish a TLC identification of Polygonum multiflorum,Caulis Spatholobi,Uncaria in 702 syrup and the content determination method of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D glucoside(referred to as stilbene glycoside)in Polygonum multiflorum to provide an reference for the quality control of 702 syrup.Methods:The components of Polygonum multiflorum,Caulis Spatholobi,Uncaria in 702 syrup were qualitatively identified by thin layer chromatography(TLC).The content of stilbene glycoside was determined by high performance liquid chromatography(HPLC).Results:The TLC spots of Polygonum multiflorum,Caulis Spatholobi,Uncaria,etc,were clear,the negative control had no interference,and had strong specificity.Stilbene glycoside showed a good linear relationship between the peak area and the range of 1.0146-25.0190μg/mL(r=0.9999),the average recovery rate was 99.6%,and the RSD was 1.58%.There was no significant difference in the content of stilbene glycoside among different batches of samples(P>0.05).Conclusion:This method can qualitatively identify 702 syrup and determine its effective components,and it has the advantages of simple operation,good repeatability and high stability,and can be used for the quality control of 702 syrup.

关 键 词：中药制剂 702糖浆 薄层色谱法 高效液相色谱法 质量标准 

分 类 号：R927.2[医药卫生—药学]