匙指虾科线粒体基因组结构与系统进化分析  

Characteristics and phylogenetic analysis of mitogenome in Atyidae

作　　者：朱陇强 朱志煌 朱雷宇 王定全 王健鑫 林琪[1] ZHU Longqiang;ZHU Zhihuang;ZHU Leiyu;WANG Dingquan;WANG Jianxin;LIN Qi(Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian Province, Xiamen 361013, China;Laboratory of Molecular Ecology and Application of Marine Microorganisms, Zhejiang Ocean University, Zhoushan 316022, China)

机构地区：[1]福建省水产研究所福建省海洋生物增养殖与高值化利用重点实验室,福建厦门361013 [2]浙江海洋大学海洋微生物分子生态与应用实验室,浙江舟山316022

出　　处：《大连海洋大学学报》2022年第3期428-434,共7页Journal of Dalian Ocean University

基　　金：国家重点研发计划“蓝色粮仓科技创新”项目(2019YFD0901305);福建省海洋与渔业结构调整专项(2020HYJG01,2020HYJG08);福建省海洋服务与渔业高质量发展专项资金项目(FJHY-YYKJ-2022-1-7);舟山市科技局科研项目(2019C21011)。

摘　　要：为深入研究匙指虾科Atyidae物种鉴定、分类及遗传进化等相关内容,利用DnaSP 6、PhyML 3.1、MEGA 5.0、MrBayes 3.2.6及BEAST 2.6.3软件对NCBI数据库中24个匙指虾科物种线粒体全基因组序列进行了分析。结果表明:匙指虾科物种线粒体基因组长度为15318~16430 bp,AT含量为62.20%~70.19%;24个匙指虾科物种线粒体基因组基因的组成和排列模式保持一致;线粒体基因组差异位点分析显示,nd5和nd4基因变异位点比例较高且变异位点数最多;基于13个蛋白质编码基因(protein-coding genes,PCGs)的核苷酸序列系统进化分析显示,除Paratyinae亚科为单系群外,Typhlatyinae和Caridellinae亚科均为非单系群,三者亲缘关系较近且聚为一大支,与单独成为一个类群的Atyinae成姊妹关系;根据分子钟估算结果推测,Paratyinae、Typhlatyinae和Caridellinae亚科物种最近的共同祖先可能出现在石炭纪中期到二叠纪时段,Atyinae亚科物种最近的共同祖先可能出现在二叠纪早期到侏罗纪时段,随后进一步分化为具有现代特征的匙指虾科种类。研究表明,nd5和nd4基因可作为匙指虾理想的分子标记进行物种遗传分析,Typhlatyinae和Caridellinae的单系性仍存在争议,地理板块运动对匙指虾科物种分化具有重要影响。In this study,the complete mitogenome sequences of 24 species in Atyidae whose members are small in size,and difficult to make the morphological identification and classification were analyzed by using DnaSP 6,PhyML 3.1,MEGA 5.0,MrBayes 3.2.6 and BEAST 2.6.3 software.The results showed that the lengths of 24 mitogenomes were shown to be changed from 15318 bp to 16430 bp,with AT content varying from 62.20%to 70.19%and consistent gene composition and arrangement of mitogenome in 24 species.The analysis of differing sites of mitogenome revealed that the ratio of variable sites nd5 and nd4 genes were relatively high,with the maximal number of variable sites.The phylogenetic trees based on 13 PCGs(protein-coding genes)showed that the subfamily of Paratyinae was a monophyletic,and that Typhlatyinae and Caridellinae were non-monophyletic which were closely related to each other and clustered into a large branch,forming a sister group with Atyinae alone.Molecular clock analysis suggested that the most recent common ancestor of species in Paratyinae,Typhlatyinae and Caridellinae might occur from the middle Carboniferous to Permian time,while the most recent common ancestor of species in Atyinae might occur from the early Permian Jurassic time,and then further differentiated into the modern characteristic species in Atyidae.In conclusion,nd5 and nd4 genes can be used as ideal molecular markers for species genetic analysis in species in Atyidae.The monophyleticity of Typhlatyinae and Caridellinae is still controversial,and the movement of geographic plates has an important impact on the species differentiation in Atyidae.

关 键 词：匙指虾科 线粒体基因组 系统发育 

分 类 号：S917[农业科学—水产科学]