不同纵横比一水草酸钙晶体的合成、表征、吸附性能及其对肾上皮细胞的毒性差异  

Synthesis, Characterization, Adsorption Properties of Calcium Oxalate Monohydrate Crystals with Different Aspect Ratios and Their Toxicity to Renal Epithelial Cell

作　　者：程小燕 徐猛 欧阳健明 CHENG Xiao-Yan;XU Meng;OUYANG Jian-Ming(Institute of Biomineralization and Lithiasis Research,College of Chemistry and Materials Science,Jinan University,Guangzhou 510632,China)

机构地区：[1]暨南大学化学与材料学院,生物矿化与结石病防治研究所,广州510632

出　　处：《无机化学学报》2022年第7期1261-1271,共11页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.21975105)资助。

摘　　要：合成了长宽比分别为1:2、1:3、1:4和1:5的4种一水草酸钙(COM)晶体COM-1:2、COM-1:3、COM-1:4和COM-1:5,采用X射线衍射、傅里叶变换红外光谱、扫描电子显微镜(SEM)、ζ电位仪和比表面测试仪等对其理化性质进行了表征。SEM图片显示晶体的宽度相近,但长度分别为(3±0.3)μm、(5.2±0.3)μm、(7.0±0.7)μm和(8.8±1.2)μm。随着反应温度升高,生成的晶体长度变长;搅拌速度越大,生成的晶体尺寸越小;随着添加剂明胶的浓度减小,COM晶体的(101)面被拉长。细胞活力、细胞总死亡率和活性氧检测表明,不同长宽比COM对人肾近端上皮细胞(HK-2)的毒性大小为COM-1:2>COM-1:3>COM-1:4>COM-1:5>对照组,SEM检测证明4种COM晶体均能够黏附到细胞表面。毒性差异与以下各原因呈正相关:晶体大比例的(101)晶面、大的比表面积、大的细胞-晶体剪切应力。Four kinds of calcium oxalate monohydrate(COM) crystals(COM-1:2,COM-1:3,COM-1:4,and COM-1:5)with the aspect ratio of 1:2,1:3,1:4,and 1:5,respectively,were synthesized.Their physicochemical properties were characterized by X-ray diffraction,Fourier transform infrared spectroscopy,scanning electron microscope(SEM),ζ potentiometer,and specific surface tester.SEM images show that the widths of the crystals were similar,but the lengths of the crystals were(3±0.3) μm,(5.2±0.3) μm,(7.0±0.7) μm,and(8.8±1.2) μm,respectively.With the increase of reaction temperature,the length of the crystal increased.The faster the stirring speed,the smaller the crystal size.As the concentration of the additive gelatin decreased,the(101) faces of COM crystals were elongated.Cell viability,cell total mortality,and reactive oxygen species(ROS) tests showed that the toxicity of COM with different length-width ratios to the human proximal tubular epithelial cells(HK-2) was COM-1:2 > COM-1:3 >COM-1:4 > COM-1:5 > control group.SEM examination showed that all four COM crystals could adhere to the cell surface.The reasons for this difference are positively related to the following points: a large proportion of (101) crystal planes,large specific surface area,and small cell-crystal shear stress.

关 键 词：一水草酸钙 尺寸效应 细胞损伤 肾结石 晶面 

分 类 号：R69[医药卫生—泌尿科学] O614.23[医药卫生—外科学]